从一位携带 TP53 基因突变的 AML 患者的 PBMC 中生成并鉴定 iPS 细胞系（SYSUSHi002-A）。
Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation.
机构信息
Pediatric Purgery Department, The University of Hong Kong Shenzhen Hospital, Shenzhen, Guangdong Province 518000, China.
Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China.
出版信息
Stem Cell Res. 2023 Dec;73:103230. doi: 10.1016/j.scr.2023.103230. Epub 2023 Oct 18.
We have generated an iPSCs line (IPS-AML2-C3, SYSUSHi002-A) from AML cells of a 71-year-old male Acute Myeloid Leukaemia patient with TP53 gene mutation (TP53: c.824G > A, p.Cys275Tyr) using episomal plasmids encoding the factors OCT4, SOX2, KLF4, L-MYC and human miR-302. The IPS-AML2-C3 (SYSUSHi002-A) iPSC line displayed typical embryonic stem cell-like morphology, carried the TP53 gene mutation, expressed several pluripotent stem cell makers, retained normal karyotype (46, XY), and was capable of forming three germ layer cells in vitro.
我们已使用编码 OCT4、SOX2、KLF4、L-MYC 和人 miR-302 的附加体质粒，从一位 71 岁的男性急性髓系白血病伴 TP53 基因突变（TP53：c.824G>A，p.Cys275Tyr）患者的 AML 细胞中生成了 iPSCs 系（IPS-AML2-C3，SYSUSHi002-A）。IPS-AML2-C3（SYSUSHi002-A）iPSC 系显示出典型的胚胎干细胞样形态，携带 TP53 基因突变，表达多种多能干细胞标志物，保持正常核型（46，XY），并能够在体外形成三个胚层细胞。
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