Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China.
Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China.
Fish Shellfish Immunol. 2023 Dec;143:109175. doi: 10.1016/j.fsi.2023.109175. Epub 2023 Oct 26.
Tumor necrosis factor (TNF) is an important cytokine that can regulate a variety of cellular responses by binding tumor necrosis factor receptor (TNFR). We studied whether the TNF of Eriocheir sinensis can regulate hemocyte proliferation. The results showed that the EsTNF and EsTNFR were constitutively expressed in all tested tissues, including the heart, hepatopancreas, muscles, gills, stomachs, intestines, and hemocytes. We found that low levels of EsTNF and EsTNFR transcripts were present in hemocytes. The gene expression levels were significantly increased in the hemocytes after being stimulated by Staphylococcus aureus or Vibrio parahaemolyticus. We also found some genes related to cell proliferation were expressed at a higher level in pulsing rTNF-stimulated hemocytes compared with the control group. We also knocked down the EsTNFR gene with RNAi technology. The results showed that the expression level of these genes related to cell proliferation was significantly down-regulated compared with the control group when the TNF does not bind TNFR. We used Edu technology to repeat the above experiments and the results were similar. Compared with the control group, the hemocytes stimulated by rTNF showed more significant proliferation, and the proliferation rate was significantly down-regulated after knocking down the EsTNFR gene. Therefore, we indicate that TNF binding TNFR can affect the proliferation of E. sinensis hemocytes, which might be manifested by affecting the expression of some proliferation-related genes.
肿瘤坏死因子（TNF）是一种重要的细胞因子，通过与肿瘤坏死因子受体（TNFR）结合，可调节多种细胞反应。我们研究了中华绒螯蟹的 TNF 是否可以调节血细胞的增殖。结果表明，EsTNF 和 EsTNFR 在所有测试的组织中均持续表达，包括心脏、肝胰腺、肌肉、鳃、胃、肠和血细胞。我们发现血细胞中存在低水平的 EsTNF 和 EsTNFR 转录本。用金黄色葡萄球菌或副溶血弧菌刺激后，血细胞中的基因表达水平显著增加。我们还发现一些与细胞增殖相关的基因在脉冲 rTNF 刺激的血细胞中的表达水平明显高于对照组。我们还使用 RNAi 技术敲低了 EsTNFR 基因。结果表明，当 TNF 不与 TNFR 结合时，与细胞增殖相关的这些基因的表达水平与对照组相比显著下调。我们使用 Edu 技术重复了上述实验，结果相似。与对照组相比，rTNF 刺激的血细胞表现出更显著的增殖，而敲低 EsTNFR 基因后，增殖率显著下调。因此，我们表明 TNF 与 TNFR 的结合可以影响中华绒螯蟹血细胞的增殖，这可能表现为影响一些增殖相关基因的表达。
