Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, United States.
Methods Cell Biol. 2023;180:39-48. doi: 10.1016/bs.mcb.2023.03.002. Epub 2023 May 13.
The extracellular vesicles (EVs) are carriers that actively transfer functional biomolecules between cells affecting the intercellular communication (Pitt, Kroemer, & Zitvogel, 2016). EV signaling has consequences on the targeted cell behavior impacting multiple processes from health to disease, including cancer (Yates et al., 2022). Radiation treatment (RT) is one of the gold standard and effective treatments for cancer, as curative or palliative (Chandra, Keane, Voncken, & Thomas, 2021). RT induces different release of EVs and their cargo is altered. In addition, the uptake of EVs secreted by irradiated cells is affected. Hence, a deep investigation is required to better understand how RT influence the cell-to-cell communication thought signals shuttle by EVs. Here, detailed methods to study the RT effects on EV size and secretion, EV protein expression, EV uptake will be described. Alterations and adaptions might make the protocols applicable to different cell lines, and with different types of RT and dose exposures.
细胞外囊泡 (EVs) 是一种载体，可在细胞间主动转移功能生物分子，从而影响细胞间通讯（Pitt、Kroemer 和 Zitvogel，2016 年）。EV 信号会影响靶细胞的行为，从而影响从健康到疾病的多种过程，包括癌症（Yates 等人，2022 年）。放射治疗 (RT) 是癌症的金标准和有效治疗方法之一，无论是作为根治性治疗还是姑息性治疗（Chandra、Keane、Voncken 和 Thomas，2021 年）。RT 诱导不同的 EV 释放，其 cargo 发生改变。此外，受辐照细胞分泌的 EV 的摄取也受到影响。因此，需要深入研究以更好地了解 RT 通过 EV 传递的信号如何影响细胞间通讯。在这里，将详细描述研究 RT 对 EV 大小和分泌、EV 蛋白表达、EV 摄取影响的方法。这些改变和适应可能会使该方案适用于不同的细胞系，并适用于不同类型的 RT 和剂量暴露。
