Department of Diagnostic Sciences, College of Dentistry, The University of Tennessee Health Science Center, Memphis, TN, USA; Department of Otolaryngology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN, USA.
Department of Diagnostic Sciences, College of Dentistry, The University of Tennessee Health Science Center, Memphis, TN, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2024 Aug;138(2):289-292. doi: 10.1016/j.oooo.2023.07.050. Epub 2023 Aug 1.
Recent studies have identified recurrent rearrangements of EWSR1 or FUS with NFATC2 in extragnathic simple bone cysts (SBCs). We investigated the presence of EWSR1 or FUS rearrangements and the immunophenotypic expression of NKX2.2 and CD99 in a series of SBCs of the jaw.
We retrieved 10 cases of SBC of the jaw from the University of Pittsburgh archives. Of the 10 cases, we were able to evaluate 8 by immunohistochemistry for CD99 and NKX2.2 and 7 by fluorescence in situ hybridization (FISH) for EWSR1 and FUS rearrangement using EWSR1 and FUS break-apart probes.
All 8 cases evaluated by immunohistochemistry expressed CD99 but were negative for NKX2.2, and all 7 cases assayed using FISH were negative for EWSR1 and FUS rearrangements.
In contrast to the SBC of extragnathic sites, we found no presence of EWSR1 and FUS rearrangements by FISH in the SBC of the jaw, suggesting that this entity may be etiologically/molecularly distinct and reflects a non-neoplastic reactive process. However, as these lesions tend to be paucicellular, FISH may not be the appropriate technique for identifying EWSR1/FUS fusions. Other techniques should be used to evaluate them in future studies.
最近的研究已经确定了 EWSR1 或 FUS 与 NFATC2 在颌外单纯性骨囊肿（SBC）中的反复重排。我们研究了一系列颌骨 SBC 中 EWSR1 或 FUS 重排以及 NKX2.2 和 CD99 的免疫表型表达。
我们从匹兹堡大学的档案中检索了 10 例颌骨 SBC。在这 10 例中，我们能够通过 CD99 和 NKX2.2 的免疫组织化学评估 8 例，通过 EWSR1 和 FUS 分离探针的荧光原位杂交（FISH）评估 7 例进行 EWSR1 和 FUS 重排。
通过免疫组织化学评估的 8 例均表达 CD99，但 NKX2.2 为阴性，通过 FISH 检测的 7 例均未发生 EWSR1 和 FUS 重排。
与颌外部位的 SBC 不同，我们通过 FISH 在颌骨 SBC 中未发现 EWSR1 和 FUS 重排，表明该实体可能在病因学/分子水平上不同，反映了一种非肿瘤性反应性过程。然而，由于这些病变往往细胞稀少，FISH 可能不是识别 EWSR1/FUS 融合的合适技术。在未来的研究中，应使用其他技术来评估它们。
