Roos K P
Am J Physiol. 1986 Dec;251(6 Pt 2):H1373-8. doi: 10.1152/ajpheart.1986.251.6.H1373.
Sarcomere lengths, cell widths, and cell volumes were determined from photomicrographs and direct optical imaging of Ca2+-tolerant myocytes dispersed from collagenase-perfused whole rat myocardium. Individual unattached cells were examined at rest first in the normosmotic Ca2+-Tyrode saline solution and then in a sequence of hypotonic or hypertonic Tyrode solutions. Myocytes swell and shrink in hypotonic and hypertonic media but not as much as expected for an ideal uniform osmotic system. Furthermore, these changes were not proportional in the longitudinal and radial directions. These data suggest an 18% osmotically inactive volume and significant internal radial and longitudinal load-bearing structures within these cardiac myocytes.
通过对从胶原酶灌注的大鼠全心肌中分离出的耐钙心肌细胞的显微照片和直接光学成像，测定肌节长度、细胞宽度和细胞体积。首先在等渗钙-台氏盐溶液中，然后在一系列低渗或高渗台氏溶液中，对单个未附着的静息细胞进行检查。心肌细胞在低渗和高渗介质中会肿胀和收缩，但程度不如理想的均匀渗透系统预期的那么大。此外，这些变化在纵向和径向上并不成比例。这些数据表明，这些心肌细胞内存在18%的渗透惰性体积以及显著的内部径向和纵向承重结构。
