Miller B R, Mitchell C J
Am J Trop Med Hyg. 1986 Nov;35(6):1302-9. doi: 10.4269/ajtmh.1986.35.1302.
The effect of successive lytic passage of yellow fever virus on mosquito infection and transmission rates in the vector, Aedes aegypti, was determined. Three strains of yellow fever virus from Trinidad and Peru were passaged five times in suckling mouse brains and seven times in BHK-21 cells. Mosquitoes were fed meals containing passaged and unpassaged viruses and infection and transmission rates were compared. Rates were similar for all but one of the three virus strains grown in both substrates with the exception of virus strain 1899/81 (human isolate from Peru) passaged seven times in BHK-21 cells. Infection rates declined from 62% (109/177) to 35% (61/176), and transmission rates declined from 64% (60/94) to 45% (22/49). The oligonucleotide fingerprint of strain 1899/81 passaged seven times in BHK-21 cells shared 98% (45/46) of its large, T1-resistant oligonucleotides with the parent strain, indicating limited biochemical differences. The data suggest that uncloned yellow fever virus populations, passaged a limited number of times, and exhibiting some phenotypic changes, are representative of the original virus strain and can be used with a reasonable degree of confidence in vector competence studies.
测定了黄热病病毒连续裂解传代对媒介埃及伊蚊的感染率和传播率的影响。来自特立尼达和秘鲁的三株黄热病病毒在乳鼠脑内传代5次，在BHK - 21细胞上传代7次。给蚊子喂食含有传代病毒和未传代病毒的食物，并比较感染率和传播率。除在BHK - 21细胞上传代7次的病毒株1899/81（来自秘鲁的人类分离株）外，在两种培养基中培养的三株病毒中，除一株外，其他病毒株的感染率和传播率相似。感染率从62%（109/177）降至35%（61/176），传播率从64%（60/94）降至45%（22/49）。在BHK - 21细胞上传代7次的1899/81株的寡核苷酸指纹图谱与其亲本株共享98%（45/46）的大的、T1抗性寡核苷酸，表明生化差异有限。数据表明，未克隆的黄热病病毒群体，传代次数有限，并表现出一些表型变化，代表了原始病毒株，可在媒介能力研究中以合理程度的置信度使用。
