Chen Changzheng, Li Kuikui, Li Tang, Li Junyan, Liu Qishun, Yin Heng
Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Technology Innovation Center for Green Agriculture, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
University of Chinese Academy of Sciences, Beijing 100190, China.
Bioengineering (Basel). 2023 Oct 21;10(10):1230. doi: 10.3390/bioengineering10101230.
Konjac glucomannan (KGM) is a natural polysaccharide derived from konjac, which has been widely used in various fields due to its numerous beneficial properties. However, the high viscosity and water absorption of KGM limit its application. Compared with KGM, Konjac glucomannan oligosaccharides (KGMOS) have higher water solubility and stronger application value. In this paper, a novel mannanase ManA was cloned from to develop a new KGMOS-producing enzyme. Bioinformatic analysis shows that the structural similarity between ManA and other enzymes was less than 18.33%. Phylogenetic analysis shows that ManA shares different branches with the traditional mannanases containing the CMB35 domain, indicating that it is a novel mannanase. Then, the enzymatic properties were determined and substrate specificity was characterized. Surprisingly, ManA is stable in a very wide pH range of 3.0 to 10.0; it has a special substrate specificity and seems to be active only for mannans without galactose in the side chain. Additionally, the three-dimensional structure of the enzyme was simulated and molecular docking of the mannotetraose substrate was performed. As far as we know, this is the first report to characterize the enzymatic properties and to simulate the structure of mannanase from . This work will contribute to the development and characterization of novel -derived mannanases. The above results indicate that ManA is a promising tool for the production of KGMOS.
魔芋葡甘聚糖（KGM）是一种从魔芋中提取的天然多糖，因其具有众多有益特性而被广泛应用于各个领域。然而，KGM的高粘度和吸水性限制了其应用。与KGM相比，魔芋葡甘聚糖寡糖（KGMOS）具有更高的水溶性和更强的应用价值。本文从[具体来源未给出]中克隆了一种新型甘露聚糖酶ManA，以开发一种新的生产KGMOS的酶。生物信息学分析表明，ManA与其他酶之间的结构相似性小于18.33%。系统发育分析表明，ManA与含有CMB35结构域的传统甘露聚糖酶分属于不同分支，表明它是一种新型甘露聚糖酶。然后，测定了其酶学性质并表征了底物特异性。令人惊讶的是，ManA在3.0至10.0的非常宽的pH范围内稳定；它具有特殊的底物特异性，似乎仅对侧链中没有半乳糖的甘露聚糖有活性。此外，模拟了该酶的三维结构并进行了甘露四糖底物的分子对接。据我们所知，这是首次对来自[具体来源未给出]的甘露聚糖酶的酶学性质进行表征并模拟其结构的报道。这项工作将有助于新型[具体来源未给出]衍生甘露聚糖酶的开发和表征。上述结果表明，ManA是生产KGMOS的一种有前途的工具。
