Karadurmus Leyla, Ozkan Sibel A
Faculty of Pharmacy, Department of Analytical Chemistry, Adıyaman University, Adıyaman 02040, Türkiye.
Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Ankara 06560, Türkiye.
Micromachines (Basel). 2023 Sep 30;14(10):1881. doi: 10.3390/mi14101881.
Molecularly Imprinted Polymers (MIP) have demonstrated considerable potential when combined with electrochemical sensors, exhibiting high sensitivity, selectivity and reproducibility levels. The aim of this work is to detect Zivudine (ZDV) in serum samples by means of an interface imprinting technique-based electrochemical sensor. Thus, ZDV was used as a template for the creation of an MIP-based electrochemical sensor, and differential pulse voltammetry (DPV) was used as the determination technique for the molecule. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were also used to characterize the electrochemical sensor capabilities, which showed a good linearity between 1.0 × 10 M and 1.0 × 10 M. ZDV was detected with a detection limit of 1.63 × 10 M, while the recovery analysis of spiked serum samples demonstrated that the sensor was highly selective.
分子印迹聚合物（MIP）与电化学传感器结合时已显示出巨大潜力，具有高灵敏度、选择性和重现性。这项工作的目的是通过基于界面印迹技术的电化学传感器检测血清样本中的齐多夫定（ZDV）。因此，ZDV被用作创建基于MIP的电化学传感器的模板，差分脉冲伏安法（DPV）被用作该分子的测定技术。电化学阻抗谱（EIS）和循环伏安法（CV）技术也用于表征电化学传感器的性能，其在1.0×10⁻⁶ M至1.0×10⁻³ M之间显示出良好的线性关系。检测到ZDV的检测限为1.63×10⁻⁷ M，而加标血清样本的回收率分析表明该传感器具有高度选择性。
