Rafati Amir Abbas, Afraz Ahmadreza
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O. Box 65174, Hamedan, Iran.
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O. Box 65174, Hamedan, Iran.
Mater Sci Eng C Mater Biol Appl. 2014 Jun 1;39:105-12. doi: 10.1016/j.msec.2014.02.037. Epub 2014 Feb 24.
The zidovudine (ZDV) is the first drug approved for the treatment of HIV virus infection. The detection and determination of this drug are very importance in human serum because of its undesirable effects. A new ZDV sensor was fabricated on the basis of nanocomposite of silver nanofilm (Ag-NF) and multiwalled carbon nanotubes (MWCNTs) immobilized on glassy carbon electrode (GCE). The modified electrodes were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), cyclic voltammetry (CV), and linear sweep voltammetry (LSV) techniques. Results showed that the electrodeposited silver has a nanofilm structure and further electrochemical studies showed that the prepared nanocomposite has high electrocatalytic activity and is appropriate for using in sensors. The amperometric technique under optimal conditions is used for the determination of ZDV ranging from 0.1 to 400ppm (0.37μM-1.5mM) with a low detection limit of 0.04ppm (0.15μM) (S/N=3) and good sensitivity. The prepared sensor possessed accurate and rapid response to ZDV and shows an average recovery of 98.6% in real samples.
齐多夫定（ZDV）是首个被批准用于治疗HIV病毒感染的药物。由于其不良影响，在人体血清中检测和测定这种药物非常重要。基于固定在玻碳电极（GCE）上的银纳米膜（Ag-NF）和多壁碳纳米管（MWCNT）的纳米复合材料制备了一种新型ZDV传感器。通过扫描电子显微镜（SEM）、能量色散X射线光谱（EDS）、X射线衍射（XRD）、循环伏安法（CV）和线性扫描伏安法（LSV）技术对修饰电极进行了表征。结果表明，电沉积银具有纳米膜结构，进一步的电化学研究表明，制备的纳米复合材料具有高电催化活性，适用于传感器。在最佳条件下，采用安培法测定ZDV，范围为0.1至400ppm（0.37μM-1.5mM），检测限低至0.04ppm（0.15μM）（S/N=3），灵敏度良好。制备的传感器对ZDV具有准确、快速的响应，在实际样品中的平均回收率为98.6%。
