Oliveira Ethiene Fernandes de, Araújo Bruno Cavalheiro, Marques Victor Hugo, de Mello Paulo Henrique, Moreira Renata Guimarães, Honji Renato Massaaki
Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo (IB/USP), Rua do Matão, trav. 14, nº 321, São Paulo 05508-090, SP, Brazil.
Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes (UMC), Mogi das Cruzes 08701-970, SP, Brazil.
Animals (Basel). 2023 Oct 14;13(20):3212. doi: 10.3390/ani13203212.
An 8-week trial was performed to evaluate the influence of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) ratios and temperature on the fatty acid (FA) metabolism and liver morphology of . A basal diet was manufactured, and DHA (D1:0.5%; D2:0.75%; D3:1%; and D4:1.5%) and EPA (D1:1.0%; D2:0.75%; D3:0.5%; and D4:0%) levels were added. were kept in twelve tanks with a lower temperature (LT, 23.17 ± 0.12 °C) and twelve with a higher temperature (HT, 28.63 ± 0.06 °C). The DHA/EPA ratio did not affect performance, regardless of the temperature, but the animals fed with the D4 diet showed better growth at HT. A higher lipid deposition and a large lipid vacuole area in the hepatocytes at HT were observed, regardless of the diet. Triacylglycerol (TG) in reflected the diet FA profile mainly in the muscle and liver, whereas the phospholipid (PL) was less influenced by the diet. The same DHA content in the TG fraction of muscle (D3 and D4) were observed at LT; however, only the DHA/EPA ratio of D4 could be differentially stored in the TG fraction of muscle (HT). Monounsaturated and polyunsaturated FA increased in the PL of the eyes at HT, whereas saturated FA was reduced in the TG and PL fractions at LT. These results evidence the importance of temperature and a balanced DHA/EPA ratio in the diet of marine fish.
进行了一项为期8周的试验，以评估二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）比例以及温度对[具体对象未提及]脂肪酸（FA）代谢和肝脏形态的影响。制备了基础饲料，并添加了不同水平的DHA（D1：0.5%；D2：0.75%；D3：1%；D4：1.5%）和EPA（D1：1.0%；D2：0.75%；D3：0.5%；D4：0%）。[具体对象未提及]被饲养在12个较低温度（LT，23.17±0.12°C）的水箱和12个较高温度（HT，28.63±0.06°C）的水箱中。无论温度如何，DHA/EPA比例均不影响生长性能，但喂食D4饲料的动物在高温下生长得更好。无论饲料如何，在高温下均观察到肝细胞中脂质沉积增加和脂质空泡面积增大。三酰甘油（TG）主要反映了肌肉和肝脏中的饲料脂肪酸谱，而磷脂（PL）受饲料的影响较小。在低温下，肌肉TG部分中观察到相同的DHA含量（D3和D4）；然而，只有D4的DHA/EPA比例在高温下可在肌肉TG部分中差异储存。在高温下，眼睛PL中的单不饱和脂肪酸和多不饱和脂肪酸增加，而在低温下，饱和脂肪酸在TG和PL部分中减少。这些结果证明了温度和饮食中平衡的DHA/EPA比例对海水鱼的重要性。
