Methner Anika, Kuzyk Steven B, Petersen Jörn, Bauer Sabine, Brinkmann Henner, Sichau Katja, Wanner Gerhard, Wolf Jacqueline, Neumann-Schaal Meina, Henke Petra, Tank Marcus, Spröer Cathrin, Bunk Boyke, Overmann Jörg
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Former Institution: Paläomikrobiologie, Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Postfach 2503, 26111 Oldenburg, Germany.
Microorganisms. 2023 Sep 25;11(10):2394. doi: 10.3390/microorganisms11102394.
In the pursuit of cultivating anaerobic anoxygenic phototrophs with unusual absorbance spectra, a purple sulfur bacterium was isolated from the shoreline of Baltrum, a North Sea island of Germany. It was designated strain 970, due to a predominant light harvesting complex (LH) absorption maximum at 963-966 nm, which represents the furthest infrared-shift documented for such complexes containing bacteriochlorophyll . A polyphasic approach to bacterial systematics was performed, comparing genomic, biochemical, and physiological properties. Strain 970 is related to DSM 6702 by 26.5, 81.9, and 98.0% similarity via dDDH, ANI, and 16S rRNA gene comparisons, respectively. The photosynthetic properties of strain 970 were unlike other spp., which contained typical LH absorbing characteristics of 800-870 nm, as well as a newly discovered absorption band at 908 nm. Strain 970 also had a different photosynthetic operon composition. Upon genomic comparisons with the original strains DSM 6702 and strain 06511, the latter was found to be divergent, with 25.3, 79.1, and 97.5% similarity via dDDH, ANI, and 16S rRNA gene homology to , respectively. Strain 06511 (=DSM 116345) is thereby described as sp. nov., and the unique strain 970 (=DSM 111777) as sp. nov.
为了培养具有异常吸收光谱的厌氧不产氧光合细菌，从德国北海岛屿巴尔图姆的海岸线分离出一种紫色硫细菌。由于其主要光捕获复合体（LH）在963 - 966 nm处有最大吸收峰，该吸收峰代表了含细菌叶绿素的此类复合体记录到的最远红外位移，因此将其命名为菌株970。采用多相方法进行细菌分类学研究，比较了基因组、生化和生理特性。通过dDDH、ANI和16S rRNA基因比较，菌株970与DSM 6702的相似性分别为26.5%、81.9%和98.0%。菌株970的光合特性与其他含有典型800 - 870 nm LH吸收特征以及新发现的908 nm吸收带的菌株不同。菌株970还具有不同的光合操纵子组成。在与原始菌株DSM 6702和菌株06511进行基因组比较时，发现后者存在差异，通过dDDH、ANI和16S rRNA基因同源性与DSM 6702的相似性分别为25.3%、79.1%和97.5%。因此，菌株06511（=DSM 116345）被描述为新种，独特的菌株970（=DSM 111777）也被描述为新种。
