Department of Food Science and Nutrition, King Saud University, Riyadh 11451, Saudi Arabia.
UniLaSalle, Univ. Artois, ULR7519-Transformations & Agro-Ressources, Normandie Université, F-76130 Mont-Saint-Aignan, France.
Molecules. 2023 Oct 11;28(20):7030. doi: 10.3390/molecules28207030.
Starches from different botanical sources are affected in the presence of enzymes. This study investigated the impact of α-amylase on several properties of pre-gelatinized starches derived from chickpea ( L.), wheat ( L.), corn ( L.), white beans (), and sweet potatoes ( L.). Specifically, the water holding capacity, freezable water content, sugar content, and water sorption isotherm (adsorption and desorption) properties were examined. The source of α-amylase utilized in this study was a germinated sorghum ( L. Moench) extract (GSE). The starch samples were subjected to annealing at temperatures of 40, 50, and 60 °C for durations of either 30 or 60 min prior to the process of gelatinization. A significant increase in the annealing temperature and GSE resulted in a notable enhancement in both the water-holding capacity and the sugar content of the starch. The ordering of starches in terms of their freezable water content is as follows: Chickpea starch (C.P.S) > white beans starch (W.B.S) > wheat starch (W.S) > chickpea starch (C.S) > sweet potato starch (S.P.S). The Guggenheim-Anderson-de Boer (GAB) model was only employed for fitting the data, as the Brunauer-Emmett-Teller (BET) model had a low root mean square error (RMSE). The application of annealing and GSE treatment resulted in a shift of the adsorption and desorption isotherms towards greater levels of moisture content. A strong hysteresis was found in the adsorption and desorption curves, notably within the water activity range of 0.6 to 0.8. The GSE treatment and longer annealing time had an impact on the monolayer water content (m), as well as the C and K parameters of the GAB model, irrespective of the annealing temperature. These results can be used to evaluate the applicability of starch in the pharmaceutical and food sectors.
来自不同植物来源的淀粉在酶的存在下会受到影响。本研究调查了α-淀粉酶对几种来源于鹰嘴豆(L.)、小麦(L.)、玉米(L.)、白豆()和甘薯(L.)的预糊化淀粉特性的影响。具体来说,研究了持水能力、可冻结水含量、糖含量以及水分吸附等温线(吸附和解吸)特性。本研究中使用的α-淀粉酶的来源是发芽高粱(L. Moench)提取物(GSE)。在糊化之前,将淀粉样品在 40、50 和 60°C 的温度下退火 30 或 60 分钟。退火温度和 GSE 的显著升高导致淀粉的持水能力和糖含量显著提高。淀粉的可冻结水含量的排序如下:鹰嘴豆淀粉(C.P.S)>白豆淀粉(W.B.S)>小麦淀粉(W.S)>鹰嘴豆淀粉(C.S)>甘薯淀粉(S.P.S)。Guggenheim-Anderson-de Boer(GAB)模型仅用于拟合数据,因为 Brunauer-Emmett-Teller(BET)模型的均方根误差(RMSE)较低。退火和 GSE 处理的应用导致吸附和解吸等温线向更高的水分含量转移。在吸附和解吸曲线中发现了强烈的滞后现象,特别是在水活度为 0.6 到 0.8 的范围内。GSE 处理和较长的退火时间对单层水分含量(m)以及 GAB 模型的 C 和 K 参数产生了影响,而与退火温度无关。这些结果可用于评估淀粉在制药和食品领域的适用性。