Department of Genetics, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Research Centre for Cultivar Testing in Słupia Wielka, Chrząstowo 8, 89-100 Nakło nad Notecią, Poland.
Int J Mol Sci. 2023 Oct 19;24(20):15349. doi: 10.3390/ijms242015349.
In this work, we present the results of the inoculation of canola seeds ( L.) with strains that promote the growth of plants. Seven morphologically different strains of (TvI-VII) were shown to be capable of synthesizing auxins and exhibited cellulolytic and pectinolytic activities. To gain a deeper insight into the molecular mechanisms underlying canola- interactions, we analyzed the canola stress genes metallothioneins () and stringent response genes ( and ). We demonstrated the presence of -regulatory elements responsive to fungal elicitors in the promoter regions of and genes and observed changes in the levels of the transcripts of the above-mentioned genes in response to root colonization by the tested fungal strains. Of the seven tested strains, under laboratory conditions, VII stimulated the formation of roots and the growth of canola seedlings to the greatest extent. An experiment conducted under field conditions during drought showed that the inoculation of canola seeds with a suspension of VII spores increased yield by 16.7%. There was also a positive effect of the fungus on the height and branching of the plants, the number of siliques, and the mass of a thousand seeds. We suggest that the strain TvVII can be used in modern sustainable agriculture as a bioinoculant and seed coating to protect from drought.
在这项工作中，我们展示了用促进植物生长的菌株接种油菜种子（ Brassica napus ）的结果。七种形态不同的根瘤菌（TvI-VII）被证明能够合成生长素，并表现出纤维素酶和果胶酶活性。为了更深入地了解油菜与根瘤菌相互作用的分子机制，我们分析了油菜胁迫基因金属硫蛋白（MTs）和严格反应基因（ SIG12 和 HSP17.6 ）。我们证明了在 和 基因的启动子区域中存在对真菌诱导剂有反应的 - 调控元件，并观察到在测试的真菌菌株根定植后，上述基因的转录本水平发生变化。在七种测试菌株中，在实验室条件下，菌株 VII 最能刺激根的形成和油菜幼苗的生长。在干旱条件下进行的田间试验表明，用 VII 孢子悬浮液接种油菜种子可使产量提高 16.7%。真菌对植物的高度和分枝、角果数和千粒重也有积极影响。我们认为，菌株 TvVII 可以在现代可持续农业中作为生物接种剂和种子包衣，以保护油菜免受干旱。
