College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory of Eye Health, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, Nanning, 530021, China.
Genes (Basel). 2023 Sep 22;14(10):1831. doi: 10.3390/genes14101831.
Circular RNAs (circRNAs) are a type of non-coding RNA that play a crucial role in the development and lactation of mammary glands in mammals. A total of 107 differentially expressed circRNAs (DE circRNAs) were found, of which 52 were up-regulated and 55 were down-regulated. We also found that DE circRNA host genes were mainly involved in GO terms related to the development process of mammary epithelial cells and KEGG pathways were mostly related to mammary epithelial cells, lactation, and gland development. Protein network analysis found that DE circRNAs can competitively bind to miRNAs as key circRNAs by constructing a circRNA-miRNA-mRNA network. CircRNAs competitively bind to miRNAs (miR-10b-3p, miR-671-5p, chi-miR-200c, chi-miR-378-3p, and chi-miR-30e-5p) involved in goat mammary gland development, mammary epithelial cells, and lactation, affecting the expression of core genes (, , , , and ). Here, we generated CiMECs and systematically explored the differences in the transcription profile for the first time using whole-transcriptome sequencing. We also analyzed the interaction among mRNA, miRNA, and cirRNA and predicted that circRNA plays an important role in the maintenance of mammary epithelial cells.
环状 RNA（circRNAs）是一种非编码 RNA，在哺乳动物乳腺的发育和泌乳中起着至关重要的作用。我们共发现 107 个差异表达的 circRNAs（DE circRNAs），其中 52 个上调，55 个下调。我们还发现，DE circRNA 宿主基因主要参与与乳腺上皮细胞发育过程相关的 GO 术语，KEGG 途径主要与乳腺上皮细胞、泌乳和腺体发育有关。蛋白网络分析发现，通过构建 circRNA-miRNA-mRNA 网络，DE circRNAs 可以作为关键 circRNAs 竞争性结合 miRNA。circRNAs 竞争性结合涉及山羊乳腺发育、乳腺上皮细胞和泌乳的 miRNA（miR-10b-3p、miR-671-5p、chi-miR-200c、chi-miR-378-3p 和 chi-miR-30e-5p），影响核心基因（、、、和）的表达。在这里，我们首次使用全转录组测序生成了 CiMECs，并系统地探索了转录谱的差异。我们还分析了 mRNA、miRNA 和 cirRNA 之间的相互作用，并预测 circRNA 在维持乳腺上皮细胞中发挥重要作用。
