Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland.
Department of Pathology, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland.
Genes (Basel). 2023 Oct 1;14(10):1903. doi: 10.3390/genes14101903.
Umbilical hernia (UH) and inguinal hernia (IH) are among the most common defects in pigs, affecting their welfare and resulting in economic losses. In this study, we aimed to verify the association of previously reported differences in transcript levels of the , , , and genes with the occurrence of UH and IH. We examined mRNA levels in muscle and connective tissue from 68 animals-34 affected by UH and 34 controls. In a second cohort, we examined inguinal channel samples from 46 pigs (in four groups). We determined DNA methylation levels in muscle tissue for the UH and control animals. The transcript level of changed in the UH cases, being upregulated and downregulated in muscle and connective tissue, respectively, and the gene also showed an increased muscular mRNA level. The transcript of the gene significantly decreased in old pigs with IH. We further observed an increased DNA methylation level for one CpG site within the gene in UH individuals. We conclude that these alterations in gene mRNA levels in the UH animals depend on the tissue and can sometimes be a consequence of, not a cause of, the affected phenotype.
脐疝（UH）和腹股沟疝（IH）是猪中最常见的缺陷之一，影响它们的福利并导致经济损失。在这项研究中，我们旨在验证先前报道的 、 、 和 基因转录水平的差异与 UH 和 IH 的发生之间的关联。我们检查了 68 只动物（34 只受 UH 影响，34 只对照）肌肉和结缔组织中的 mRNA 水平。在第二组中，我们检查了 46 只猪（分为四组）的腹股沟通道样本。我们确定了 UH 和对照动物肌肉组织中的 DNA 甲基化水平。在 UH 病例中， 的转录水平发生改变，分别在肌肉和结缔组织中上调和下调，并且 基因也显示出肌肉 mRNA 水平增加。IH 老年猪中 基因的转录显著降低。我们还观察到 UH 个体中 基因内一个 CpG 位点的 DNA 甲基化水平增加。我们的结论是，UH 动物中这些基因 mRNA 水平的改变取决于组织，有时可能是受影响表型的结果，而不是原因。
