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丁香酚：体外和卵内评估以探究其对骨肉瘤和口咽癌细胞的细胞毒性作用

Eugenol: In Vitro and In Ovo Assessment to Explore Cytotoxic Effects on Osteosarcoma and Oropharyngeal Cancer Cells.

作者信息

Racea Robert-Cosmin, Macasoi Ioana-Gabriela, Dinu Stefania, Pinzaru Iulia, Marcovici Iasmina, Dehelean Cristina, Rusu Laura-Cristina, Chioran Doina, Rivis Mircea, Buzatu Roxana

机构信息

Faculty of Dental Medicine, "Victor Babeș" University of Medicine and Pharmacy from Timisoara, 9 Revolutiei 1989 Ave., 300070 Timisoara, Romania.

Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, "Victor Babeș" University of Medicine and Pharmacy from Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.

出版信息

Plants (Basel). 2023 Oct 12;12(20):3549. doi: 10.3390/plants12203549.

DOI:10.3390/plants12203549
PMID:37896013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10610311/
Abstract

Cancer is a significant health problem worldwide; consequently, new therapeutic alternatives are being investigated, including those found in the vegetable kingdom. Eugenol (Eug) has attracted attention for its therapeutic properties, especially in stomatology. The purpose of this study was to investigate the cytotoxicity of Eug, in vitro, on osteosarcoma (SAOS-2) and oropharyngeal squamous cancer (Detroit-562) cells, as well as its potential irritant effect in ovo at the level of the chorioallantoic membrane (CAM). The data obtained following a 72 h Eug treatment highlighted the reduction in cell viability up to 41% in SAOS-2 cells and up to 37% in Detroit-562 cells, respectively. The apoptotic-like effect of Eug was indicated by the changes in cell morphology and nuclear aspect; the increase in caspase-3/7, -8 and -9 activity; the elevated expression of Bax and Bad genes; and the increase in luminescence signal (indicating phosphatidylserine externalization) that preceded the increase in fluorescence signal (indicating the compromise of membrane integrity). Regarding the vascular effects, slight signs of coagulation and vascular lysis were observed, with an irritation score of 1.69 for Eug 1 mM. Based on these results, the efficiency of Eug in cancer treatment is yet to be clarified.

摘要

癌症是全球范围内一个重大的健康问题；因此，人们正在研究新的治疗方法，包括从植物界发现的那些方法。丁香酚（Eug）因其治疗特性而受到关注，尤其是在口腔医学领域。本研究的目的是在体外研究丁香酚对骨肉瘤（SAOS-2）细胞和口咽鳞状细胞癌（Detroit-562）细胞的细胞毒性，以及其在鸡胚尿囊膜（CAM）水平上的潜在刺激作用。丁香酚处理72小时后获得的数据突出显示，SAOS-2细胞的细胞活力分别降低了41%，Detroit-562细胞的细胞活力分别降低了37%。丁香酚的凋亡样作用表现为细胞形态和细胞核形态的变化；半胱天冬酶-3/7、-8和-9活性增加；Bax和Bad基因表达升高；以及在荧光信号增加（表明膜完整性受损）之前发光信号增加（表明磷脂酰丝氨酸外化）。关于血管效应，观察到轻微的凝血和血管溶解迹象，1 mM丁香酚的刺激评分为1.69。基于这些结果，丁香酚在癌症治疗中的有效性尚待阐明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a59/10610311/afc1a32e5e7f/plants-12-03549-g011.jpg
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