Bandick Rasmus, Busmann Lia V, Mousavi Soraya, Shayya Nizar W, Piwowarski Jakub P, Granica Sebastian, Melzig Matthias F, Bereswill Stefan, Heimesaat Markus M
Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, Germany.
Microbiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, 02-097 Warsaw, Poland.
Pharmaceutics. 2023 Oct 1;15(10):2410. doi: 10.3390/pharmaceutics15102410.
Human food-borne infections with the enteropathogen are becoming increasingly prevalent worldwide. Since antibiotics are usually not indicated in campylobacteriosis, alternative treatment regimens are important. We here investigated potential disease-alleviating effects of menthol and of extracts from tormentil, raspberry leaves, and loosestrife in acute murine campylobacteriosis. Therefore, -infected microbiota-depleted IL-10 mice were orally treated with the compounds alone or all in combination from day 2 until day 6 post-infection. Whereas neither treatment regimen affected gastrointestinal pathogen loads, the combination of compounds alleviated -induced diarrheal symptoms in diseased mice on day 6 post-infection. Furthermore, the therapeutic application of tormentil and menthol alone and the combination of the four compounds resulted in lower colonic T cell numbers in infected mice when compared to placebo counterparts. Notably, pro-inflammatory cytokines measured in mesenteric lymph nodes taken from -infected mice following tormentil, menthol, and combination treatment did not differ from basal concentrations. However, neither treatment regimen could dampen extra-intestinal immune responses, including systemic pro-inflammatory cytokine secretion on day 6 post-infection. In conclusion, the combination of menthol and of extracts from tormentil, raspberry leaves, and loosestrife constitutes an antibiotic-independent approach to alleviate campylobacteriosis symptoms.
人类因肠道病原体引起的食源性感染在全球范围内日益普遍。由于弯曲杆菌病通常不使用抗生素治疗，因此替代治疗方案很重要。我们在此研究了薄荷醇以及委陵菜、覆盆子叶和珍珠菜提取物在急性小鼠弯曲杆菌病中的潜在疾病缓解作用。因此，在感染后第2天至第6天，对感染的微生物群耗尽的IL-10小鼠单独或联合口服这些化合物。虽然两种治疗方案均未影响胃肠道病原体载量，但化合物组合在感染后第6天减轻了患病小鼠由弯曲杆菌引起的腹泻症状。此外，与安慰剂组相比，单独使用委陵菜和薄荷醇以及四种化合物的组合进行治疗，可使感染小鼠的结肠T细胞数量减少。值得注意的是，在接受委陵菜、薄荷醇及联合治疗的感染小鼠的肠系膜淋巴结中测得的促炎细胞因子与基础浓度无差异。然而，两种治疗方案均无法抑制包括感染后第6天全身促炎细胞因子分泌在内的肠外免疫反应。总之，薄荷醇与委陵菜、覆盆子叶和珍珠菜提取物的组合构成了一种不依赖抗生素的缓解弯曲杆菌病症状的方法。
