Achayuthakan Piyada, Wongsagonsup Rungtiwa, Sriprablom Jiratthitikan, Suphantharika Manop, Intra Panich
Food Industrial Microbiology and Bioinnovation Program, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Dusit, Bangkok 10300, Thailand.
Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand.
Polymers (Basel). 2023 Oct 14;15(20):4087. doi: 10.3390/polym15204087.
The effect of pulsed electric field (PEF) treatment depends mainly on the electric field strength and treatment time. In this study, wheat flour-water suspensions were treated with PEF at an electric field strength of 3 kV/cm for 0 to 1400 pulses to obtain a specific energy input of 0 to 656 kJ/kg. The effect of PEF on the removal or unfolding of proteins from the starch surface, digestibility, starch granule structure, and physicochemical properties of wheat flour was studied. The removal of proteins from the surface and the damage to the internal structure of wheat starch granules after PEF treatment was detected by confocal laser scanning microscopy (CLSM) and FTIR. The damage of the PEF-treated wheat starch granules was observed by scanning electron microscopy (SEM). From CLSM results, penetration of dextran (Mw 10,000 Da) into starch granules of wheat flour was dependent on the energy input of PEF. The high the energy input showed the intense penetration of the biopolymer. The benefits of the accessibility of biopolymer in starch granules are to increase enzyme digestion, especially rapidly digestible starch (RDS). The RDS of wheat flour treated with PEF at 656 kJ/kg was 41.72%, whereas the RDS of wheat flour control was 27.59%.
脉冲电场（PEF）处理的效果主要取决于电场强度和处理时间。在本研究中，将小麦粉 - 水悬浮液在3 kV/cm的电场强度下用PEF处理0至1400个脉冲，以获得0至656 kJ/kg的比能量输入。研究了PEF对小麦粉中蛋白质从淀粉表面去除或展开、消化率、淀粉颗粒结构以及理化性质的影响。通过共聚焦激光扫描显微镜（CLSM）和傅里叶变换红外光谱（FTIR）检测PEF处理后小麦淀粉颗粒表面蛋白质的去除情况以及内部结构的损伤。通过扫描电子显微镜（SEM）观察PEF处理的小麦淀粉颗粒的损伤情况。从CLSM结果来看，葡聚糖（分子量10,000 Da）进入小麦粉淀粉颗粒的程度取决于PEF的能量输入。能量输入越高，生物聚合物的渗透越强烈。生物聚合物在淀粉颗粒中的可及性的好处是增加酶消化，特别是快速消化淀粉（RDS）。在656 kJ/kg下用PEF处理的小麦粉的RDS为41.72%，而对照小麦粉的RDS为27.59%。
