Trop Biomed. 2023 Sep 1;40(3):331-336. doi: 10.47665/tb.40.3.009.
Immune responses are largely regulated by cytokines. Genetic polymorphisms of the regulatory coding regions are recognized to impact the expression of cytokines. The abnormal cytokine levels in hepatitis C virus (HCV) infection seems to be involved in disease progression, viral survival, and therapeutic response. The current study assesses the polymorphisms associated with IL-6, IL-10, IL28B, IFN-γ, TGF-β, and TNF-α on the genotypic susceptibility to HCV infection and Ribavirin response to Peg interferon. Droplet digital polymerase chain reaction (PCR) was used to assess the gene polymorphisms associated with IL-6 A/G (rs2069837), IL-10-1082 G/A (rs1800896)], IL28B C/T (rs12979860), IFN-γ +874 A/T (rs2430561), TGF-β 1-509 C/T (rs1800469) and TNF-α-308 G/A promoter (rs1800629) from stored samples of 200 healthy individuals and 300 HCV infected patients. There was a significant association of AG and AA genotypes of IL28B, IFN-γ, TGF-β1, and TNF-α over HCV susceptibility and treatment outcome. However, no association between IL-6 and IL-10 gene polymorphism to HCV susceptibility response to the treatment. The observations indicate IL28B CT, TGF-β1 CT, TT and TNF- AG with AA genotypes influence the cytokine expression, which is related to susceptibility and resistance to HCV infection and combined antiviral therapy.
免疫反应在很大程度上受到细胞因子的调节。已经认识到调节编码区的遗传多态性会影响细胞因子的表达。丙型肝炎病毒 (HCV) 感染中异常的细胞因子水平似乎与疾病进展、病毒存活和治疗反应有关。本研究评估了与 IL-6、IL-10、IL28B、IFN-γ、TGF-β 和 TNF-α 相关的基因多态性对 HCV 感染的基因型易感性和聚乙二醇干扰素联合利巴韦林的反应。液滴数字聚合酶链反应 (PCR) 用于评估与 IL-6 A/G (rs2069837)、IL-10-1082 G/A (rs1800896)、IL28B C/T (rs12979860)、IFN-γ+874 A/T (rs2430561)、TGF-β1-509 C/T (rs1800469) 和 TNF-α-308 G/A 启动子 (rs1800629) 相关的基因多态性，这些基因多态性与 200 名健康个体和 300 名 HCV 感染患者的储存样本相关。IL28B、IFN-γ、TGF-β1 和 TNF-α 的 AG 和 AA 基因型与 HCV 易感性和治疗结果显著相关。然而，IL-6 和 IL-10 基因多态性与 HCV 易感性对治疗的反应之间没有关联。这些观察结果表明，IL28B CT、TGF-β1 CT、TT 和 TNF-AG 与 AA 基因型影响细胞因子表达，这与 HCV 感染和联合抗病毒治疗的易感性和耐药性有关。
