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六个品种对干旱胁迫的生理响应及抗旱性

[Physiological response to drought stress and drought resistance of six cultivars].

作者信息

Zhao Lei, Jin Hai-di, Cao Xiao-Yun, Deng Wen-Hui, DU Ling-Juan

机构信息

College of Landscape Architecture, Northwest A&F University, Yangling 712100, Shaanxi, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2023 Oct;34(10):2644-2654. doi: 10.13287/j.1001-9332.202310.003.

Abstract

We examined the responses of physiological and leaf anatomic structural characteristics of six cultivars to different degrees of drought stress. A membership function was used to evaluate drought resis-tance and identify physiological and leaf anatomical indicators that exhibited a stronger correlation with drought tolerance. The results showed that leaf thickness, leaf area per unit mass and soluble protein levels of the six cultivars significantly decreased with the increases of drought stress. Net photosynthetic rate, stomatal conductance, and transpiration rate of leaves increased first and then decreased, while the intercellular CO concentration decreased. The relative electrical conductivity, MDA, and HO contents of leaves were increased. Soluble saccharide and proline contents, and antioxidant enzyme activities were first elevated and then decreased. With the increases of drought stress, the ratio of palisade tissue thickness to sponge tissue thickness and stomatal density increased. Key indicators and relativities in evaluating drought resistance of those cultivars were proline, soluble sugars, and the ratio of palisade tissue thickness to sponge tissue thickness. 'Anemone Red' and 'Ane-mone Red spotted' had better drought resistance, which could be the excellent parental materials for the cultivation of new drought-resistant cultivars in the future.

摘要

我们研究了6个品种的生理和叶片解剖结构特征对不同程度干旱胁迫的响应。采用隶属函数法评价其抗旱性,并确定与耐旱性相关性较强的生理和叶片解剖指标。结果表明,随着干旱胁迫程度的增加,6个品种的叶片厚度、单位质量叶面积和可溶性蛋白含量均显著降低。叶片净光合速率、气孔导度和蒸腾速率先升高后降低,而胞间CO₂浓度降低。叶片的相对电导率、丙二醛和H₂O₂含量增加。可溶性糖和脯氨酸含量以及抗氧化酶活性先升高后降低。随着干旱胁迫程度的增加栅栏组织厚度与海绵组织厚度的比值和气孔密度增加。评价这些品种抗旱性的关键指标及相关性为脯氨酸、可溶性糖以及栅栏组织厚度与海绵组织厚度的比值。‘秋牡丹红’和‘斑叶秋牡丹红’具有较好的抗旱性,有望成为未来培育抗旱新品种的优良亲本材料。

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