Department of Animal Science, University of Wyoming, Laramie, Wyoming, USA.
United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, Texas, USA.
J Environ Sci Health B. 2023;58(12):711-717. doi: 10.1080/03601234.2023.2273754. Epub 2023 Nov 2.
Foodborne pathogen has been associated with ruminants. The objectives of this experiment were to determine survivability in mixed rumen microbial populations and the impact on methane production with or without methane inhibitors 2-bromosulfonate (BES) and/or sodium nitrate. When inoculated into rumen microbial populations without or with 0.5 mM BES, 5.0 mM nitrate or their combination, viability decreased from 4.7 ± 0.1 log colony forming units (CFU)/mL after 24 h. Loss of viability was greater ( < 0.05) when incubated under 100% CO compared to 50% H:50% CO, decreasing 1.46 1.15 log units, respectively. viability was also decreased ( < 0.05) by more than 0.43 log units by the anti-methanogen treatments. Rumen microbial populations produced less methane ( = 0.05) when incubated with than without regardless of whether under 100% CO or 50% H:50% CO. For either gas phase, nitrate was decreased (13.2 37.9%) by the anti-methanogen treatments versus controls although not always significant. -inoculated populations metabolized 16.4% more ( < 0.05) nitrate under H:CO 100% CO. Apparently, can compete for H with methanogens but has limited survivability under rumen conditions.
食源性病原体与反刍动物有关。本实验的目的是确定在混合瘤胃微生物群中的存活能力，以及有无甲烷抑制剂 2-溴磺酸盐（BES）和/或硝酸钠对甲烷生成的影响。当接种到没有或含有 0.5 mM BES、5.0 mM 硝酸盐或它们的组合的瘤胃微生物群中时，在 24 小时后，活度从 4.7 ± 0.1 对数集落形成单位（CFU）/mL 降低。与 50%H:50%CO 相比，在 100%CO 下孵育时，活度的损失更大（<0.05），分别降低了 1.46 和 1.15 对数单位。抗甲烷菌处理也使活度降低了超过 0.43 对数单位（<0.05）。无论在 100%CO 还是 50%H:50%CO 下，与不接种相比，接种的微生物群产生的甲烷减少（=0.05）。对于任何一种气体相，抗甲烷菌处理与对照相比，硝酸盐减少（13.2-37.9%），尽管并不总是显著的。在 H:CO 下，接种的种群比在 100%CO 下代谢更多的硝酸盐（16.4%）（<0.05）。显然，能够与产甲烷菌竞争 H，但在瘤胃条件下存活能力有限。
