• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

斑马鱼脑注册与评价 (BREEZE)-映射：全脑结构和活性分析的流水线。

Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping: A pipeline for whole-brain structural and activity analyses.

机构信息

Child Study Center, Yale School of Medicine, New Haven, CT 06510, USA; Department of Neuroscience, Yale University, New Haven, CT 06510, USA; Interdepartmental Neuroscience Program, Yale School of Medicine, New Haven, CT 06510, USA; Department of Neurology, Yale School of Medicine, New Haven, CT 06510, USA.

Child Study Center, Yale School of Medicine, New Haven, CT 06510, USA.

出版信息

STAR Protoc. 2023 Dec 15;4(4):102647. doi: 10.1016/j.xpro.2023.102647. Epub 2023 Oct 26.

DOI:10.1016/j.xpro.2023.102647
PMID:37897734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10641303/
Abstract

Here, we present Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping, a user-friendly pipeline for the registration and analysis of whole-brain images in larval zebrafish. We describe steps for pre-processing, registration, quantification, and visualization of whole-brain phenotypes in zebrafish mutants of genes associated with neurodevelopmental and neuropsychiatric disorders. By utilizing BioImage Suite Web, an open-source software package originally developed for processing human brain imaging data, we provide a highly accessible whole-brain mapping protocol developed for users with general computational proficiency. For complete details on the use and execution of this protocol, please refer to Weinschutz Mendes et al. (2023)..

摘要

在这里，我们展示了用于斑马鱼的大脑注册和评估（BREEZE）映射，这是一个用户友好的流水线，用于注册和分析神经发育和神经精神疾病相关基因的斑马鱼幼虫的全脑图像。我们描述了使用 BioImage Suite Web（最初是为处理人类大脑成像数据而开发的开源软件包）进行预处理、注册、量化和可视化斑马鱼突变体全脑表型的步骤。为了让具有一般计算能力的用户能够使用，我们提供了一个高度可访问的全脑映射协议。有关使用和执行此协议的完整详细信息，请参阅 Weinschutz Mendes 等人（2023）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/3d7864a902f5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/318c8f86ee92/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/0e0735530844/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/08c3b90bf6d8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/41800b32ff91/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/23ce89eb5e79/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/e3813c4499c9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/3d7864a902f5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/318c8f86ee92/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/0e0735530844/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/08c3b90bf6d8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/41800b32ff91/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/23ce89eb5e79/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/e3813c4499c9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1871/10641303/3d7864a902f5/gr6.jpg

相似文献

1
Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping: A pipeline for whole-brain structural and activity analyses.斑马鱼脑注册与评价 (BREEZE)-映射：全脑结构和活性分析的流水线。
STAR Protoc. 2023 Dec 15;4(4):102647. doi: 10.1016/j.xpro.2023.102647. Epub 2023 Oct 26.
2
Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy.使用全组织免疫荧光和光片显微镜技术在斑马鱼幼体大脑中可视化pRps6阳性细胞的实验方案。
STAR Protoc. 2025 Mar 21;6(1):103587. doi: 10.1016/j.xpro.2024.103587. Epub 2025 Jan 18.
3
Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing.幼体斑马鱼的机械性脊髓横断术和随后的全器官组织学处理。
STAR Protoc. 2022 Jan 17;3(1):101093. doi: 10.1016/j.xpro.2021.101093. eCollection 2022 Mar 18.
4
Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes.通过计算平均斑马鱼淋巴管共聚焦图像来识别表型的方案。
STAR Protoc. 2025 Mar 21;6(1):103632. doi: 10.1016/j.xpro.2025.103632. Epub 2025 Feb 8.
5
A protocol for simultaneous Ca and morphology imaging of brain endothelial tip cells in larval zebrafish.用于在幼年斑马鱼中同时对脑内皮尖端细胞的 Ca 和形态成像的方案。
STAR Protoc. 2021 Mar 18;2(1):100388. doi: 10.1016/j.xpro.2021.100388. eCollection 2021 Mar 19.
6
Bi-channel image registration and deep-learning segmentation (BIRDS) for efficient, versatile 3D mapping of mouse brain.双通道图像配准和深度学习分割（BIRDS）用于高效、通用的小鼠大脑 3D 映射。
Elife. 2021 Jan 18;10:e63455. doi: 10.7554/eLife.63455.
7
Visualization and quantitation of spine deformity in zebrafish models of scoliosis by micro-computed tomography.微计算机断层扫描技术在脊柱侧凸斑马鱼模型中脊柱畸形的可视化和定量分析。
STAR Protoc. 2023 Dec 15;4(4):102739. doi: 10.1016/j.xpro.2023.102739. Epub 2023 Dec 2.
8
Protocol for controlling visual experience during zebrafish development and modulation of motor behavior.斑马鱼发育过程中视觉体验控制及运动行为调制方案。
STAR Protoc. 2023 Dec 15;4(4):102636. doi: 10.1016/j.xpro.2023.102636. Epub 2023 Oct 13.
9
Brain Imaging and Registration in Larval Zebrafish.脑成像和幼虫斑马鱼的配准
Methods Mol Biol. 2024;2707:141-153. doi: 10.1007/978-1-0716-3401-1_9.
10
Protocol for image-based small-molecule screen to identify neuroprotective compounds for dopaminergic neurons in zebrafish.基于图像的小分子筛选以鉴定斑马鱼多巴胺能神经元神经保护化合物的方案。
STAR Protoc. 2024 Mar 15;5(1):102837. doi: 10.1016/j.xpro.2024.102837. Epub 2024 Jan 12.