Department of Oral and Maxillofacial Pathology, College of Dentistry, University of Baghdad, Baghdad, Iraq.
J Med Life. 2023 Jul;16(7):1022-1027. doi: 10.25122/jml-2022-0312.
Various malignant and benign tumors can arise in the sinonasal cavity, including inverted papilloma (IP), a benign neoplasm with unique clinical characteristics. However, the mechanisms involved in the recurrence, occurrence, and malignant transformation of IP remain debatable. This study aimed to investigate the impact of human papillomavirus (HPV) infections on IP by comparing the number of infections in cases with epithelial tissue dysplasia and explore the predictive role of proliferative and prognostic markers in dysplasia. Tissue blocks from 35 cases of sinonasal papilloma, collected between 2015 and 2021 from the laboratory archives of the Medical City of Ghazi Al-Hererri Hospital in Baghdad, Iraq, were immunohistochemically stained with monoclonal antibodies (mAbs) to detect Ki-67 and p53. A quantitative immunohistochemical analysis was conducted to analyze the results. Polymerase chain reaction (PCR) was performed to detect HPV genotypes 16/18 and 6/11 in the tissues. There was an insignificant increase in Ki-67 and p53 expression in inverted papillomas with dysplasia. HPV11 was the most prevalent genotype in 34.3% of the patients, followed by HPV16 and HPV18 in 31.4% of the patients for each virus. The least common virus detected was human papillomavirus 6 (8.6%), which did not show any significant association with the degree of dysplasia. Viral detection proliferation and apoptosis had no impact on tumor dysplasia amongst all the patients, showing no relationship with the evaluated cases.
各种恶性和良性肿瘤都可发生于鼻窦腔，包括内翻性乳头状瘤（IP），这是一种具有独特临床特征的良性肿瘤。然而，涉及 IP 的复发、发生和恶性转化的机制仍存在争议。本研究旨在通过比较具有上皮组织异型增生的病例中感染的数量，来研究人乳头瘤病毒（HPV）感染对内翻性乳头状瘤的影响，并探讨增殖和预后标志物在异型增生中的预测作用。从 2015 年至 2021 年，从伊拉克巴格达医疗城 Ghazi Al-Hererri 医院的实验室档案中收集了 35 例鼻窦乳头状瘤组织块，并用单克隆抗体（mAbs）对 Ki-67 和 p53 进行免疫组织化学染色，以检测 HPV 感染。进行了定量免疫组化分析来分析结果。对组织进行聚合酶链反应（PCR）以检测 HPV 基因型 16/18 和 6/11。具有异型增生的内翻性乳头状瘤中 Ki-67 和 p53 的表达略有增加。HPV11 是最常见的基因型，在 34.3%的患者中存在，HPV16 和 HPV18 在 31.4%的患者中各存在一种。检测到的最不常见的病毒是人乳头瘤病毒 6（8.6%），其与异型增生的程度没有任何显著关联。病毒检测增殖和凋亡对所有患者的肿瘤异型增生没有影响，与评估病例无关。
