ENAH-202 通过调节 ZNF502/VIM 轴促进口腔鳞状细胞癌的进展。
ENAH-202 promotes cancer progression in oral squamous cell carcinoma by regulating ZNF502/VIM axis.
机构信息
School of Stomatology, Weifang Medical University, Weifang, Shandong, China.
Weifang Key Laboratory of Oral Biomedicine, Weifang Medical University, Weifang, Shandong, China.
出版信息
Cancer Med. 2023 Nov;12(22):20892-20905. doi: 10.1002/cam4.6652. Epub 2023 Oct 30.
BACKGROUND
We aimed to demonstrate the regulatory effect of long non-coding RNA (lncRNA) ENAH-202 on oral squamous cell carcinoma (OSCC) development as well as its molecular mechanism.
METHODS
We detected ENAH-202 expression in OSCC tissues and cell lines by quantitative real-time PCR (qPCR). The biological function of ENAH-202 was assessed in vitro and in vivo using CCK-8, colony formation assays, transwell assays, xenograft formation, and tail vein injection. The further molecular mechanism by which ENAH-202 promoted OSCC progression was identified using RNA pull-down, LS-MS/MS analysis, RNA immunoprecipitation (RIP), and chromatin immunoprecipitation (ChIP) assays.
RESULTS
ENAH-202 was significantly upregulated in OSCC tissues and cells. ENAH-202 promoted OSCC cell proliferation, migration, and invasion in vitro and in vivo. The expression of enabled homolog (ENAH) and epithelial-to-mesenchymal transition (EMT)-related proteins was changed with the expression of ENAH-202. Moreover, ENAH-202 promoted the transcription of Vimentin (VIM) by binding with ZNF502, which can help ENAH-202 promote OSCC progression.
CONCLUSIONS
ENAH-202 facilitated OSCC cell proliferation and metastasis by regulating ZNF502/VIM axis, which played an important role in OSCC progression.
背景
本研究旨在探讨长链非编码 RNA (lncRNA) ENAH-202 对口腔鳞状细胞癌 (OSCC) 发展的调控作用及其分子机制。
方法
采用实时定量 PCR (qPCR) 检测 OSCC 组织和细胞系中 ENAH-202 的表达。通过 CCK-8、集落形成实验、Transwell 实验、异种移植形成和尾静脉注射实验，在体外和体内评估 ENAH-202 的生物学功能。通过 RNA 下拉、LS-MS/MS 分析、RNA 免疫沉淀 (RIP) 和染色质免疫沉淀 (ChIP) 实验鉴定 ENAH-202 促进 OSCC 进展的进一步分子机制。
结果
ENAH-202 在 OSCC 组织和细胞中显著上调。ENAH-202 促进 OSCC 细胞在体外和体内的增殖、迁移和侵袭。ENAH-202 的表达改变了 enabled homolog (ENAH) 和上皮间质转化 (EMT) 相关蛋白的表达。此外，ENAH-202 通过与 ZNF502 结合促进 Vimentin (VIM) 的转录，从而有助于 ENAH-202 促进 OSCC 的进展。
结论
ENAH-202 通过调节 ZNF502/VIM 轴促进 OSCC 细胞的增殖和转移，在 OSCC 进展中发挥重要作用。
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