Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo, 11562, Egypt.
Bioanalysis. 2023 Nov;15(22):1327-1341. doi: 10.4155/bio-2023-0138. Epub 2023 Oct 30.
An eco-friendly ultra-performance liquid chromatography-tandem mass spectrometry method was developed to study the pharmacokinetics of rivaroxaban and ticagrelor in rat plasma, utilizing moxifloxacin as an internal standard. The food-drug interaction between grapefruit juice and these drugs was also investigated. Liquid-liquid extraction was used. A nonporous stationary phase Agilent Poroshell 120EC C18 column was used with methanol: 0.1% aqueous formic acid (95:5 v/v) as a mobile phase. The detection was performed in multiple reaction monitoring mode using positive electrospray ionization. The method's validation was conducted in accordance with US FDA and European Medicines Agency guidelines. Grapefruit juice should be ingested with caution in patients treated with antithrombotic medications as it may increase their plasma concentration, inducing bleeding, and requires close clinical monitoring.
建立了一种超高效液相色谱-串联质谱法，以研究利伐沙班和替格瑞洛在大鼠血浆中的药代动力学，并用莫西沙星作为内标。还研究了柚子汁与这些药物之间的食物-药物相互作用。采用液-液萃取法。使用非多孔固定相 Agilent Poroshell 120EC C18 柱，以甲醇：0.1%甲酸水溶液（95:5v/v）为流动相。采用正电喷雾电离多反应监测模式进行检测。该方法的验证符合美国食品和药物管理局和欧洲药品管理局的指南。在接受抗血栓药物治疗的患者中，应谨慎摄入柚子汁，因为它可能会增加其血浆浓度，导致出血，需要密切的临床监测。
