Department of Immunology, Institute of Medicine, University of Tsukuba, Tsukuba, Japan.
Department of Otolaryngology-Head and Neck Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan.
Monoclon Antib Immunodiagn Immunother. 2023 Oct;42(5):182-185. doi: 10.1089/mab.2023.0017.
CD300A is a member of the CD300 immunoglobulin (Ig)-like receptor family consisting of eight molecules in humans, all of which contain one Ig-like domain in the extracellular portion. Upon binding its ligand phosphatidylserine or phosphatidylethanolamine, CD300A mediates an inhibitory signal through the immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic portion. The CD300 family molecules are highly homologous to each other. In addition, CD300A has a single nucleotide polymorphism (rs2272111), which is a nonsense mutation encoding glutamine (CD300A) instead of arginine (CD300A) at residue 111 in the Ig-like domain of CD300A. In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300A or CD300A or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.
CD300A 是 CD300 免疫球蛋白 (Ig) 样受体家族的成员之一，人类共有 8 个分子，它们都在细胞外部分含有一个 Ig 样结构域。CD300A 与其配体磷脂酰丝氨酸或磷脂酰乙醇胺结合后，通过细胞质部分的免疫受体酪氨酸抑制基序传递抑制信号。CD300 家族分子彼此高度同源。此外，CD300A 有一个单核苷酸多态性 (rs2272111)，这是一个无义突变，导致第 111 位氨基酸残基由精氨酸 (CD300A) 变为谷氨酰胺 (CD300A)，该突变位于 CD300A 的 Ig 样结构域中。在这项研究中，我们成功地生成了针对 CD300A 或 CD300A 或两者的单克隆抗体 (mAb)。这些 mAb 可用于通过流式细胞术分析 CD300A 基因型，并开发用于治疗各种疾病的抗体药物。
