Department of Agricultural Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
J Appl Microbiol. 2023 Nov 1;134(11). doi: 10.1093/jambio/lxad247.
Microbial biocontrol agents have become an effective option to mitigate the harmfulness of chemical pesticides in recent years. This study demonstrates the control efficacy of Bacillus velezensis CE 100 on the anthracnose causal agent, Colletotrichum gloeosporioides.
In vitro antifungal assays revealed that the culture filtrate and volatile organic compounds of B. velezensis CE 100 strongly restricted the mycelial development of C. gloeosporioides. Moreover, a bioactive compound, butyl succinate, was isolated from the n-butanol crude extract of B. velezensis CE 100 (bce), and identified by liquid chromatography-electrospray ionization hybrid ion-trap and time-of-flight mass spectrometry (LC-ESI-QTOF-MS) and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR). Treatment with purified butyl succinate at a concentration of 300 μg mL-1 strongly controlled conidial germination of C. gloeosporioides with an inhibition rate of 98.66%, whereas butyl succinate at a concentration of 400 μg mL-1 showed weak antifungal action on the mycelial growth of C. gloeosporioides with an inhibition rate of 31.25%. Scanning electron microscopy revealed that the morphologies of butyl succinate-treated hyphae and conidia of C. gloeosporioides were severely deformed with shriveled and wrinkled surfaces. Furthermore, butyl succinate was able to control carbendazim-resistant C. gloeosporioides, demonstrating that it could be a promising agent for the suppression of other carbendazim-resistant fungal pathogens. An in vivo biocontrol assay demonstrated that the strain ce 100 broth culture and butyl succinate showed higher control efficacy on apple anthracnose than bce.
Our findings provide insight into the antifungal potential of B. velezensis ce 100 and its butyl succinate for efficient control of phytopathogenic fungi, such as C. gloeosporiodes, in plant disease protection. This is the first study to demonstrate the antifungal potential of bacteria-derived butyl succinate for control of C. gloeosporioides.
近年来，微生物生物防治剂已成为减轻化学农药危害的有效选择。本研究展示了芽孢杆菌 CE100 对炭疽病病原菌胶孢炭疽菌的控制效果。
体外抗真菌试验表明，芽孢杆菌 CE100 的培养滤液和挥发性有机化合物强烈抑制了胶孢炭疽菌的菌丝生长。此外，从芽孢杆菌 CE100 的正丁醇粗提物中分离出一种活性化合物丁酸丁酯，并通过液相色谱-电喷雾串联离子阱和飞行时间质谱（LC-ESI-QTOF-MS）以及一维（1D）和二维（2D）核磁共振（NMR）进行了鉴定。用浓度为 300μg mL-1 的纯化丁酸丁酯处理可强烈控制胶孢炭疽菌分生孢子的萌发，抑制率为 98.66%，而浓度为 400μg mL-1 的丁酸丁酯对胶孢炭疽菌菌丝生长的抑菌作用较弱，抑制率为 31.25%。扫描电子显微镜显示，丁酸丁酯处理的胶孢炭疽菌菌丝和分生孢子的形态严重变形，表面皱缩。此外，丁酸丁酯能够控制多菌灵抗性胶孢炭疽菌，表明它可能是抑制其他多菌灵抗性真菌病原体的有前途的药剂。体内生物防治试验表明，菌株 ce100 菌液培养物和丁酸丁酯对苹果炭疽病的防治效果高于 bce。
我们的研究结果为芽孢杆菌 CE100 及其丁酸丁酯对植物病原菌如胶孢炭疽菌的抑菌潜力提供了深入了解，为植物病害防治提供了一种有效的方法。这是首次研究细菌衍生的丁酸丁酯对控制胶孢炭疽菌的抑菌潜力。
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