College of Innovation and Management, Songkhla Rajabhat University, Songkhla, Thailand.
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hatyai, Thailand.
J Appl Microbiol. 2021 Sep;131(3):1452-1463. doi: 10.1111/jam.15037. Epub 2021 Mar 5.
Application of volatile organic compounds (VOCs) from Streptomyces salmonis PSRDC-09 (VOCs PSRDC-09) grown on sterile wheat seeds against chili anthracnose pathogen was investigated in vitro and in vivo.
Among 10 isolates of Colletotrichum species tested, Colletotrichum gloeosporioides PSU-03 was selected as the most aggressive anthracnose pathogenic strain on chili fruit against VOCs produced by Streptomyces species. Among 11 isolates, the strain PSRDC-09 exhibited the highest antifungal activity and was identified as S. salmonis PSRDC-09. The antagonistic mechanism of the VOCs PSRDC-09 on morphological of C. gloeosporioides PSU-03, observed by scanning electron microscope (SEM), revealed the irregular distortions in the fungal hyphae. The effect of inoculum size and spore concentration of S. salmonis PSRDC-09 prepared as a wheat seed inoculum on the suppression of C. gloeosporioides PSU-03 was studied both in vitro and on chili fruit. The optimum inoculum size (45 g 0·31 l ) and spore concentration (10 spores per ml) of the wheat seed culture of S. salmonis PSRDC-09 exhibited the complete suppression (100% inhibition) on C. gloeosporioides PSU-03. The optimum fumigation period of the VOCs PSRDC-09 (45 g 1·38 l ) was found to be 24 h. Based on gas chromatography-mass spectrometry (GC-MS) analysis, 14 major VOCs (produced by the strain PSRDC-09) were detected and l-linalool was the main volatile component.
The results indicated that the VOCs from S. salmonis PSRDC-09 could effectively control the chili anthracnose disease caused by C. gloeosporioides.
These findings suggest that S. salmonis PSRDC-09 may have the potential to become a promising biofumigant for biocontrol of chili anthracnose disease in the postharvest system.
研究了鲑鱼链霉菌 PSRDC-09（PSRDC-09 VOCs）在无菌小麦种子上生长产生的挥发性有机化合物（VOCs）对辣椒炭疽病病原菌的体外和体内应用。
在所测试的 10 个炭疽菌分离株中，选择炭疽菌 PSU-03 作为对辣椒果实最具侵袭性的炭疽病病原菌，以测试链霉菌产生的 VOCs。在 11 个分离株中，PSRDC-09 菌株表现出最高的抗真菌活性，被鉴定为鲑鱼链霉菌 PSRDC-09。扫描电子显微镜（SEM）观察到 PSRDC-09 VOCs 对 PSU-03 真菌菌丝形态的拮抗机制，显示真菌菌丝不规则扭曲。研究了作为小麦种子接种物制备的鲑鱼链霉菌 PSRDC-09 的接种物大小和孢子浓度对 PSU-03 抑制的影响，包括在体外和辣椒果实上。鲑鱼链霉菌 PSRDC-09 小麦种子培养的最佳接种物大小（45 g 0.31 l）和孢子浓度（10 个孢子/ml）对 PSU-03 表现出完全抑制（100%抑制）。发现 PSRDC-09 VOCs 的最佳熏蒸期（45 g 1.38 l）为 24 h。基于气相色谱-质谱联用（GC-MS）分析，检测到 14 种主要 VOCs（由 PSRDC-09 菌株产生），其中 l-芳樟醇是主要挥发性成分。
结果表明，鲑鱼链霉菌 PSRDC-09 的 VOCs 可有效控制炭疽菌引起的辣椒炭疽病。
这些发现表明，鲑鱼链霉菌 PSRDC-09 可能具有成为采后系统中防治辣椒炭疽病的有前途的生物熏蒸剂的潜力。
