Department of Urology, The Second People's Hospital of Kunshan, Kunshan, Jiangsu, China.
Immun Inflamm Dis. 2023 Oct;11(10):e1061. doi: 10.1002/iid3.1061.
To uncover the effects of chrysosplenol D (CHD) on the progression of prostate cancer in vitro as well as in mice.
DU145 and PC-3 cells were treated with increasing doses of CHD for 24, 48, or 72 h. Cell Counting Kit-8 (CCK-8) and colony formation assays were conducted to confirm the effects of CHD on cell viability. Flow cytometry (FCM) and immunostaining assays showed the effects of CHD on cell apoptosis and oxidative stress. Immunoblot was performed to detect the effects of CHD on autophagy. Besides, tumor growth assays were conducted to confirm the role of CHD in tumor growth in mice. GraphPad 6.0 was used for statistical analysis. All data were represented as mean ± SD. p < .05 and the significant difference was indicated by an asterisk.
CHD suppressed the viability of prostate cancer cells in CCK-8 assays, decreased colony number in colony formation assays, and induced cell apoptosis in FCM and immunostaining assays. CHD also restrained the oxidative stress with a decreased 2'-7'-dichlorofluorescein diacetate staining intensity. CHD restrained the autophagy of prostate cancer cells, as well as suppressed tumor growth in mice.
CHD could serve as a drug for prostate cancer.
在体外和小鼠体内揭示 chrysosplenol D（CHD）对前列腺癌进展的影响。
用递增剂量的 CHD 处理 DU145 和 PC-3 细胞 24、48 或 72 小时。通过细胞计数试剂盒-8（CCK-8）和集落形成实验来确认 CHD 对细胞活力的影响。流式细胞术（FCM）和免疫染色实验显示 CHD 对细胞凋亡和氧化应激的影响。免疫印迹用于检测 CHD 对自噬的影响。此外，进行肿瘤生长实验以确认 CHD 在小鼠肿瘤生长中的作用。使用 GraphPad 6.0 进行统计分析。所有数据均表示为均值±标准差。p<.05 并通过星号表示显著差异。
CHD 在 CCK-8 实验中抑制前列腺癌细胞的活力，在集落形成实验中减少集落数量，并在 FCM 和免疫染色实验中诱导细胞凋亡。CHD 还通过降低 2'-7'-二氯荧光素二乙酸染色强度来抑制氧化应激。CHD 抑制前列腺癌细胞的自噬，并抑制小鼠的肿瘤生长。
CHD 可作为治疗前列腺癌的药物。
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