Department of Medicine, Albert Einstein College of Medicine, New York, New York, USA.
Department of Pathology, Albert Einstein College of Medicine, New York, New York, USA.
mSphere. 2023 Dec 20;8(6):e0048223. doi: 10.1128/msphere.00482-23. Epub 2023 Oct 31.
Laura Cheney works at the crossroads of HIV and autophagy, a critical biological process for cellular homeostasis, to understand more completely the pathogenesis of HIV-associated neurocognitive impairment. In this mSphere of Influence article, she reflects on how "A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag" by Willa Wen-You Yim, Hayashi Yamamoto, and Noboru Mizushima (eLife 11:e78923, 2022, https://doi.org/10.7554/eLife.78923) expands the tools for studying autophagy and inspired her to use this technology to develop a reporter to study autophagy of mitochondria, termed mitophagy, to further her own research goals.
劳拉·切尼(Laura Cheney)的工作涉及 HIV 和自噬的交叉领域,自噬是细胞内稳态的关键生物学过程,她希望能更全面地了解 HIV 相关神经认知障碍的发病机制。在这篇 mSphere 影响力文章中,她反思了威拉·温友·伊姆(Willa Wen-You Yim)、林原雅史(Hayashi Yamamoto)和水岛伸夫(Noboru Mizushima)的“用于哺乳动物自噬通量的脉冲可检测报告基因处理测定法与 HaloTag”(eLife 11:e78923, 2022, https://doi.org/10.7554/eLife.78923)一文如何扩展了研究自噬的工具,并激发她使用这项技术开发一种报告基因来研究线粒体自噬,即 mitophagy,以推进她自己的研究目标。