Institut de l'Audition, Institut Pasteur, Université de Paris, INSERM, Paris, France.
Institut de l'Audition, Institut Pasteur, Université de Paris, INSERM, Paris, France.
STAR Protoc. 2023 Dec 15;4(4):102669. doi: 10.1016/j.xpro.2023.102669. Epub 2023 Oct 30.
Training mice to perform perceptual tasks is a vital part of integrative neuroscience. Replacing classical rewards like water with medial forebrain bundle (MFB) stimulation allows experimenters to avoid deprivation and obtain higher trial numbers per session. Here, we provide a protocol for implementing MFB-based reward in mice. We describe steps for MFB electrode implantation, efficacy testing, and stimulation calibration. After these steps, MFB reward can be used to facilitate sensory discrimination task training and enable nuanced characterization of psychophysical abilities. For complete details on the use and execution of this protocol, please refer to Verdier et al. (2022)..
训练老鼠执行感知任务是整合神经科学的重要组成部分。用中脑束（MFB）刺激代替经典的奖励，如饮水，可以使实验者避免剥夺并在每次实验中获得更多的试验次数。在这里，我们提供了一个在老鼠身上实施 MFB 奖励的方案。我们描述了 MFB 电极植入、功效测试和刺激校准的步骤。在这些步骤之后，MFB 奖励可以用来促进感觉辨别任务的训练，并能够细致地描述心理物理能力。如需完整的使用和执行本方案的详细信息，请参考 Verdier 等人（2022 年）。
