Hochner-Celnikier D, Marandici A, Iohan F, Monder C
Biol Reprod. 1986 Oct;35(3):633-40. doi: 10.1095/biolreprod35.3.633.
The estrogen and progesterone receptors of several organs of the prenatal cynomolgus macaque and the fetal mouse were studied using a combination of the dextran-coated charcoal technique and high-performance liquid chromatography. This procedure permitted the concurrent measurement of both receptors in minute amounts of tissue. Estrogen receptors, but not progesterone receptors, were found in the fetal monkey and mouse uteri. No estrogen or progesterone receptors were detected in the lungs, liver, kidney, heart, brain, adrenal gland, or limbs of mouse or monkey fetuses. The nonspecific binding of radioactive ORG-2058 was not displaced by unlabeled progesterone, 17 alpha-hydroxyprogesterone caproate, or ORG-2058. Because the steroid receptors that are indispensable mediators of steroid hormone action were absent from the nonreproductive tissues, prenatal development of these organs and tissues cannot be adversely influenced by exposure to estradiol, progesterone, or their synthetic analogues.
运用葡聚糖包被活性炭技术与高效液相色谱法相结合的方法，对产前食蟹猕猴和胎鼠多个器官的雌激素和孕激素受体进行了研究。该方法能够同时测定微量组织中的两种受体。在胎猴和胎鼠的子宫中发现了雌激素受体，但未发现孕激素受体。在小鼠或猴胎儿的肺、肝、肾、心脏、脑、肾上腺或四肢中未检测到雌激素或孕激素受体。放射性ORG - 2058的非特异性结合不能被未标记的孕激素、己酸17α - 羟孕酮或ORG - 2058所取代。由于非生殖组织中不存在作为类固醇激素作用不可或缺介质的类固醇受体，因此这些器官和组织的产前发育不会受到雌二醇、孕激素或其合成类似物暴露的不利影响。
