Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia; email:
Annu Rev Biophys. 2024 Jul;53(1):1-18. doi: 10.1146/annurev-biophys-030722-111729. Epub 2024 Jun 28.
Protein-protein association and aggregation are fundamental processes that play critical roles in various biological phenomena, from cellular signaling to disease progression. Understanding the underlying biophysical principles governing these processes is crucial for elucidating their mechanisms and developing strategies for therapeutic intervention. In this review, we provide an overview of recent experimental studies focused on protein-protein association and aggregation. We explore the key biophysical factors that influence these processes, including protein structure, conformational dynamics, and intermolecular interactions. We discuss the effects of environmental conditions such as temperature, pH and related buffer-specific effects, and ionic strength and related ion-specific effects on protein aggregation. The effects of polymer crowders and sugars are also addressed. We list the techniques used to study aggregation. We analyze emerging trends and challenges in the field, including the development of computational models and the integration of multidisciplinary approaches for a comprehensive understanding of protein-protein association and aggregation.
蛋白质-蛋白质的相互作用和聚集是基本过程，在各种生物现象中发挥着关键作用，从细胞信号传递到疾病进展。了解控制这些过程的基本物理原理对于阐明其机制和开发治疗干预策略至关重要。在这篇综述中，我们提供了最近关注蛋白质-蛋白质相互作用和聚集的实验研究的概述。我们探讨了影响这些过程的关键物理因素，包括蛋白质结构、构象动力学和分子间相互作用。我们讨论了环境条件（如温度、pH 和相关缓冲特定效应，以及离子强度和相关离子特定效应）对蛋白质聚集的影响。聚合物拥挤物和糖的影响也得到了讨论。我们列出了用于研究聚集的技术。我们分析了该领域的新兴趋势和挑战，包括计算模型的发展以及多学科方法的整合，以全面了解蛋白质-蛋白质的相互作用和聚集。
