Kuznetsov I A, Fillipov S M
Biofizika. 1979 May-Jun;24(3):542-4.
Conductance of the DNA isoionic solution at different degrees of neutralization has been investigated at 25 degrees. There are regions on the conductometric titration curve corresponding to titration of: 1) primary phosphoryl groups 2) guanine 3) adenine 4) adenine and cytosine in half-protonated pairs 5) thymine. The pH intervals corresponding to protonation of bases in isoionic solutions are shifted into alkaline region compared with Na-DNA solutions. It has also been found for molar per cent of unprotonatad bases to equal to that of thymine determined as from conductometric titration curve so from chromatographic analysis of the DNA hydrolysate.
在25摄氏度下研究了不同中和度的DNA等离子溶液的电导率。在电导滴定曲线上存在对应于以下滴定的区域：1）伯磷酰基；2）鸟嘌呤；3）腺嘌呤；4）半质子化对中的腺嘌呤和胞嘧啶；5）胸腺嘧啶。与Na-DNA溶液相比，等离子溶液中碱基质子化对应的pH区间向碱性区域移动。还发现，从未质子化碱基的摩尔百分比来看，根据电导滴定曲线以及DNA水解产物的色谱分析确定的胸腺嘧啶摩尔百分比是相等的。
