全基因组鉴定和表达分析揭示了组蛋白甲基转移酶和去甲基化酶基因在马铃薯（Solanum tuberosum L.）热应激反应中的作用。

Genome-wide identification and expression analysis reveal the role of histone methyltransferase and demethylase genes in heat stress response in potato (Solanum tuberosum L.).

机构信息

Integrative Plant AdaptOmics Lab (iPAL), Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India.

出版信息

Biochim Biophys Acta Gen Subj. 2024 Jan;1868(1):130507. doi: 10.1016/j.bbagen.2023.130507. Epub 2023 Nov 2.

DOI:10.1016/j.bbagen.2023.130507

PMID:37925032

Abstract

BACKGROUND

Potato (Solanum tuberosum L.), the third most important non-cereal crop, is sensitive to high temperature. Histone modifications have been known to regulate various abiotic stress responses. However, the role of histone methyltransferases and demethylases remain unexplored in potato under heat stress.

METHODS

Potato genome database was used for genome-wide analysis of StPRMT and StHDMA gene families, which were further characterized by analyzing gene structure, conserved motif, domain organization, sub-cellular localization, promoter region and phylogenetic relationships. Additionally, expression profiling under high-temperature stress in leaf and stolon tissue of heat contrasting potato genotypes was done to study their role in response to high temperature stress.

RESULTS

The genome-wide analysis led to identification of nine StPRMT and eleven StHDMA genes. Structural analysis, including conserved motifs, exon/intron structure and phylogenetic relationships classified StPRMT and StHDMA gene families into two classes viz. Class I and Class II. A variety of cis-regulatory elements were explored in the promoter region associated with light, developmental, hormonal and stress responses. Prediction of sub-cellular localization of StPRMT proteins revealed their occurrence in nucleus and cytoplasm, whereas StHDMA proteins were observed in different sub-cellular compartments. Furthermore, expression profiling of StPRMT and StHDMA gene family members revealed genes responding to heat stress. Heat-inducible expression of StPRMT1, StPRMT3, StPRMT4 and StPRMT5 in leaf and stolon tissues of HS and HT cultivar indicated them as probable candidates for enhancing thermotolerance in potato. However, StHDMAs responded dynamically in leaf and stolon tissue of heat contrasting genotypes under high temperature.

CONCLUSION

The current study presents a detailed analysis of histone modifiers in potato and indicates their role as an important epigenetic regulators modulating heat tolerance.

GENERAL SIGNIFICANCE

Understanding epigenetic mechanisms underlying heat tolerance in potato will contribute towards breeding of thermotolerant potato varieties.

摘要

背景

马铃薯（Solanum tuberosum L.）是第三大重要的非谷类作物，对高温敏感。组蛋白修饰已被证明可调节各种非生物胁迫反应。然而，在热胁迫下，马铃薯中组蛋白甲基转移酶和去甲基酶的作用仍未被探索。

方法

利用马铃薯基因组数据库对 StPRMT 和 StHDMA 基因家族进行了全基因组分析，通过分析基因结构、保守基序、结构域组织、亚细胞定位、启动子区和系统发育关系进一步对其进行了特征描述。此外，还对热对比马铃薯基因型叶片和匍匐茎组织在高温胁迫下的表达谱进行了分析，以研究它们在应对高温胁迫中的作用。

结果

全基因组分析鉴定出了 9 个 StPRMT 和 11 个 StHDMA 基因。结构分析，包括保守基序、外显子/内含子结构和系统发育关系，将 StPRMT 和 StHDMA 基因家族分为两类，即 I 类和 II 类。在与光、发育、激素和应激反应相关的启动子区域探索了多种顺式调控元件。StPRMT 蛋白的亚细胞定位预测表明，它们存在于细胞核和细胞质中，而 StHDMA 蛋白存在于不同的亚细胞区室中。此外，StPRMT 和 StHDMA 基因家族成员的表达谱分析显示，这些基因对热应激有反应。HS 和 HT 品种叶片和匍匐茎组织中 StPRMT1、StPRMT3、StPRMT4 和 StPRMT5 的热诱导表达表明它们可能是增强马铃薯耐热性的候选基因。然而，StHDMAs 在热对比基因型的叶片和匍匐茎组织中对高温表现出动态响应。