Huizhou Institute for Occupational Health, Huizhou, China; Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang, China; Liaoning Key Laboratory of Obesity and Glucose/Lipid Associated Metabolic Diseases, Shenyang, China.
Department of Pediatrics, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
J Nutr Biochem. 2024 Feb;124:109516. doi: 10.1016/j.jnutbio.2023.109516. Epub 2023 Nov 2.
Vitamin D has received increasing attention because of its association with atopic disease development. Limited studies that have been done on the impact of maternal vitamin D levels during pregnancy on infantile eczema are still debatable. We wanted to discover the effect of maternal vitamin D on infantile eczema and explore whether regulatory T cells (Treg) play a role in this process. 219 pairs of mothers and children were enrolled. Maternal fasting venous blood was collected in pregnancy's second and third trimesters to determine vitamin D levels. Cord blood and placenta samples were collected during childbirth for detecting levels of genes, proteins and cytokines. Pediatricians followed up the prevalence of eczema in infants within 1 year. The reported rate of vitamin D deficiency and insufficiency was 35.6% and 28.3%. Lower maternal 25(OH)D levels were related to a higher risk of infantile eczema. Foxp3 gene expression is lower in cord blood of infants with eczema compared to infants without eczema. There was a positive correlation between maternal 25(OH)D levels and the expression of FOXP3 gene in cord blood. Compared to vitamin D sufficiency women, vitamin D deficiency women's placental FOXP3 protein expression was decreased and PI3K/AKT/mTOR protein was up-regulated. Our study demonstrates that low prenatal maternal vitamin D levels increased the risk of infantile eczema aged 0-1 year, which might be related to the downregulating of the FOXP3 gene expression in cord blood and decreased placental FOXP3 protein expression. Low placental FOXP3 protein was related with activating PI3K/AKT/mTOR signaling pathway.
维生素 D 因其与特应性疾病发展的相关性而受到越来越多的关注。关于孕期母体维生素 D 水平对婴儿湿疹的影响的有限研究仍存在争议。我们想探讨母体维生素 D 对婴儿湿疹的影响，并研究调节性 T 细胞（Treg）是否在此过程中发挥作用。我们纳入了 219 对母婴。在妊娠的第二和第三个三个月采集母亲空腹静脉血以确定维生素 D 水平。在分娩时采集脐血和胎盘样本以检测基因、蛋白质和细胞因子的水平。儿科医生在 1 年内随访婴儿湿疹的患病率。报告的维生素 D 缺乏和不足率分别为 35.6%和 28.3%。母体 25(OH)D 水平较低与婴儿湿疹的风险增加相关。与无湿疹的婴儿相比，患有湿疹的婴儿脐血中的 Foxp3 基因表达较低。脐带血中 25(OH)D 水平与 FOXP3 基因的表达呈正相关。与维生素 D 充足的女性相比，维生素 D 缺乏的女性胎盘 FOXP3 蛋白表达减少，PI3K/AKT/mTOR 蛋白表达上调。我们的研究表明，产前母体维生素 D 水平较低会增加 0-1 岁婴儿湿疹的风险，这可能与脐带血中 FOXP3 基因表达下调和胎盘 FOXP3 蛋白表达减少有关。低胎盘 FOXP3 蛋白与激活 PI3K/AKT/mTOR 信号通路有关。
