Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, 06591, South Korea.
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, 06591, South Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, 06591, South Korea.
Biochem Biophys Res Commun. 2023 Dec 17;686:149183. doi: 10.1016/j.bbrc.2023.149183. Epub 2023 Oct 30.
Dysregulation of gene expression is critical for the progression of cancer. The augmented expression of hnRNP A1 in patients with hepatocellular carcinoma (HCC) has been related to its oncogenic functions. However, the underlying mechanisms responsible for upregulation of hnRNP A1 have not been fully elucidated. In the present study, we identified microRNA-195-5p (miR-195-5p), a miRNA downregulated in HCC, as a novel regulator governing hnRNP A1 expression. Notably, our investigations showed an inverse correlation between hnRNP A1 level, which was increased in HCC, and miR-195-5p level, which was decreased. Our findings demonstrated that hnRNP A1 significantly enhanced the migration and invasion of PLC/PRF/5 cells through its association with mRNAs regulating metastasis. MiR-195-5p also interfered with the hnRNP A1-mediated cell migration by targeting hnRNP A1. Our results underscore the significance of the miR-195-5p/hnRNP A1 axis in regulating the migratory potential of cancer cells and its role in promoting HCC by orchestrating cell migration processes.
基因表达失调对癌症的进展至关重要。肝癌（HCC）患者中 hnRNP A1 的表达增加与其致癌功能有关。然而，导致 hnRNP A1 上调的潜在机制尚未完全阐明。在本研究中，我们确定了 microRNA-195-5p（miR-195-5p），一种在 HCC 中下调的 miRNA，作为调控 hnRNP A1 表达的新型调节剂。值得注意的是，我们的研究表明 hnRNP A1 水平与 miR-195-5p 水平呈负相关，hnRNP A1 水平在 HCC 中增加，而 miR-195-5p 水平则降低。我们的研究结果表明，hnRNP A1 通过与调节转移的 mRNA 结合，显著增强了 PLC/PRF/5 细胞的迁移和侵袭。miR-195-5p 还通过靶向 hnRNP A1 干扰 hnRNP A1 介导的细胞迁移。我们的研究结果强调了 miR-195-5p/hnRNP A1 轴在调节癌细胞迁移潜能中的重要性，以及通过协调细胞迁移过程在促进 HCC 中的作用。
