Department of Surgery, University of North Carolina, Chapel Hill, NC, 27599, USA.
Department of Surgery, University of North Carolina, Chapel Hill, NC, 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, 27599, USA.
Biochem Biophys Res Commun. 2023 Dec 17;686:149200. doi: 10.1016/j.bbrc.2023.149200. Epub 2023 Oct 31.
Stem/progenitor cell therapy is a promising treatment option for patients with type 1 diabetes (T1D) a disease characterized by autoimmune destruction of pancreatic β cells. Actively injecting cells into an organ is one option for cell delivery, but in the pancreas, this contributes to acute inflammation and pancreatitis. We employed a patch grafting approach to transplant biliary tree stem cells/progenitor cells (BTSC) onto the surface of the pancreas in diabetic mice. The cells engraft and differentiate into β-like cells reversing hyperglycemia during a four-month period of observation. In addition, C-peptide and insulin gradually increase in blood circulation without detectable adverse effects during this period. Moreover, the patch graft transplant promoted the proliferation and differentiation of pancreatic β-like cells with co-expression of the β cell biomarker. CONCLUSION: BTSC transplantation can effectively attenuate T1D over a four-month period that is vital important for clinical applications.
干细胞/祖细胞治疗是 1 型糖尿病 (T1D) 患者的一种有前途的治疗选择，该疾病的特征是自身免疫破坏胰腺β细胞。将细胞主动注入器官是细胞递送的一种选择，但在胰腺中，这会导致急性炎症和胰腺炎。我们采用贴片移植方法将胆管树干细胞/祖细胞 (BTSC) 移植到糖尿病小鼠的胰腺表面。在四个月的观察期内，细胞植入并分化为β样细胞，逆转高血糖。此外，在这段时间内，C 肽和胰岛素逐渐增加到血液循环中，没有可检测到的不良反应。此外，贴片移植促进了胰腺β样细胞的增殖和分化，并共同表达β细胞生物标志物。结论：BTSC 移植可以在四个月内有效缓解 T1D，这对临床应用至关重要。
