Bekasova O D, Kashentseva E M, Evstigneev V B
Biol Bull Acad Sci USSR. 1978 Jul-Aug;5(4):437-42.
In connection with the elucidation of the possibility of photochemical participation of phycobiliproteins in the primary processes of photosynthesis, the ability of a mixture of phycocyanin + allophycocyanin (PC + APC) for photosensitization of redox reactions in the adsorbed state was investigated. It was shown that adsorbates of PC + APC on Sephadexes G-200 and G-25, diethylaminoethylcellulose, carboxyethylcellulose, Bacto-agar, Lifogel, polyethylene glycol, Dowex 50Wx2, and aluminum oxide are capable of sensitizing the photoreduction of methyl red by ascorbid acid. In this case the effectiveness of the sensitizing action depends on the concentration of the adsorbate, the pigment concentration on the carrier, the pH of the medium, and the nature of the solvent. It was shown that in the case of binding to a carrier, the sensitizing ability of PC + APC increases in comparison with that for pigments in the dissolved state. It is suggested that this is promoted by an increase in the concentration and a mutual approach of the reagents after adsorption, and the possible formation of complexes of some or all the participants of the reaction on the surface of the adsorbent.
关于阐明藻胆蛋白在光合作用初级过程中光化学参与的可能性，研究了藻蓝蛋白+别藻蓝蛋白混合物（PC + APC）在吸附状态下对氧化还原反应的光敏化能力。结果表明，PC + APC在葡聚糖凝胶G - 200和G - 25、二乙氨基乙基纤维素、羧乙基纤维素、细菌琼脂、Lifogel、聚乙二醇、Dowex 50Wx2和氧化铝上的吸附物能够敏化抗坏血酸对甲基红的光还原反应。在这种情况下，敏化作用的有效性取决于吸附物的浓度、载体上色素的浓度、介质的pH值和溶剂的性质。结果表明，与溶解状态的色素相比，PC + APC与载体结合时的敏化能力增强。这表明吸附后试剂浓度的增加和相互靠近，以及吸附剂表面上部分或所有反应参与者可能形成的复合物促进了这种情况。
