[烟酸及其衍生物对鸡肝乙酰辅酶A羧化酶活性的抑制作用]
[Inhibition of activity of acetyl-CoA-carboxylase from chicken liver by nicotinic acid and its derivatives].
作者信息
Fomenko A I, Shushevich S I, Khalmuradov A G
出版信息
Biokhimiia. 1979 Jun;44(6):1005-9.
Nicotinic acid and nicotinamide inhibit in vitro the acetyl-CoA-carboxylase activity of partially purified enzyme from chicken liver. The incorporation of 10, 20, 50 and 100 mkmoles of nicotinic acid or nicotinamide into the incubation medium (0,9 ml) leads to the inhibition of the enzyme activity by 19, 45, 70 and 100% and by 39, 51, 60 and 78%, respectively. NADH+ and NADP+ at concentrations by one order of magnitude lower than those of nicotinic acid and nicotinamide decrease the enzyme activity in a similar manner. The constants of inhibition by the above-mentioned compounds were calculated with respect to ATP, acetyl-CoA and citrate.
烟酸和烟酰胺在体外可抑制鸡肝部分纯化酶的乙酰辅酶A羧化酶活性。在孵育培养基（0.9毫升）中加入10、20、50和100微摩尔的烟酸或烟酰胺，分别可导致酶活性被抑制19%、45%、70%和100%以及39%、51%、60%和78%。浓度比烟酸和烟酰胺低一个数量级的NADH⁺和NADP⁺以类似方式降低酶活性。针对ATP、乙酰辅酶A和柠檬酸计算了上述化合物的抑制常数。
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