Boondaeng Antika, Vaithanomsat Pilanee, Apiwatanapiwat Waraporn, Trakunjae Chanaporn, Janchai Phornphimon, Suriyachai Nopparat, Kreetachat Torpong, Wongcharee Surachai, Imman Saksit
Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, Thailand.
School of Energy and Environment, University of Phayao, Tambon Maeka, Amphur Muang, Phayao 56000, Thailand.
ACS Omega. 2023 Oct 17;8(43):40433-40441. doi: 10.1021/acsomega.3c05004. eCollection 2023 Oct 31.
Agricultural waste is an alternative source for plant growth regulator biosynthesis by microorganisms. Actinobacteria are important soil microbes that significantly impact the soil as plant growth-promoting rhizobacteria and biofertilizers. This study focused on developing low-cost medium based on bagasse to improve indole-3-acetic acid (IAA) production by BS50-1 using a response surface methodology (RSM). Among 34 actinobacterial strains, BS50-1 produced the highest IAA level within the selected medium. An RSM based on a central composite design optimized the appropriate nutrients for IAA production. Thus, glucose hydrolysate and l-tryptophan at concentrations of 3.55 and 5.0 g/L, respectively, were the optimal factors that improved IAA production from 37.50 to 159.47 μg/mL within 168 h. This study reported a potential application of leftover bagasse as the raw material for cultivating actinobacteria, which efficiently produce IAA to promote plant growth.
农业废弃物是微生物合成植物生长调节剂的替代来源。放线菌是重要的土壤微生物，作为促进植物生长的根际细菌和生物肥料，对土壤有显著影响。本研究着重于开发基于甘蔗渣的低成本培养基，以利用响应面法（RSM）提高BS50-1菌株的吲哚-3-乙酸（IAA）产量。在34株放线菌菌株中，BS50-1在选定培养基中产生的IAA水平最高。基于中心复合设计的响应面法优化了IAA生产的适宜营养成分。因此，葡萄糖水解物和L-色氨酸浓度分别为3.55 g/L和5.0 g/L时，是在168小时内将IAA产量从37.50 μg/mL提高到159.47 μg/mL的最佳因素。本研究报道了剩余甘蔗渣作为培养放线菌的原料的潜在应用，放线菌可有效产生IAA以促进植物生长。
