使用新型基于挤出的 3D 打印技术 SMART 进行脂质-质粒 DNA 颗粒的概念验证准备
A Proof-of-Concept Preparation of Lipid-Plasmid DNA Particles Using Novel Extrusion-Based 3D-Printing Technology, SMART.
机构信息
Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712, United States.
Pharmaceutical Engineering and 3D printing (PharmE3D) Lab, Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, Oxford, Mississippi 38677, United States.
出版信息
Mol Pharm. 2023 Dec 4;20(12):6504-6508. doi: 10.1021/acs.molpharmaceut.3c00783. Epub 2023 Nov 6.
Gene therapy is a promising approach with delivery of mRNA, small interference RNA, and plasmid DNA to elicit a therapeutic action using cationic or ionizable lipid nanoparticles. In the present study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) developed in-house was employed for the preparation, characterization, and transfection abilities of the green fluorescence protein (GFP) plasmid DNA in cancer cells . The results showed 100% encapsulation of pDNA (GFP) in LNPs of around 150 nm (N/P 5) indicating that the processes developed using SMART technology are consistent and can be utilized for commercial applications.
基因治疗是一种很有前途的方法，它通过阳离子或可离子化脂质纳米粒将 mRNA、小干扰 RNA 和质粒 DNA 递送到细胞内，从而发挥治疗作用。本研究采用了一种新型的基于挤出的喷雾多吸附滴再沉淀技术（SMART），该技术由本实验室自主开发，用于制备、表征和转染癌症细胞中的绿色荧光蛋白（GFP）质粒 DNA。结果表明，LNPs（N/P 5）中约 150nm 的 pDNA（GFP）的包封率达到了 100%，这表明使用 SMART 技术开发的工艺是一致的，可以用于商业应用。
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