2,3,5-三苯基氯化四氮唑作为抗非结核分枝杆菌药敏试验的比色指示剂。
2,3,5-triphenyl tetrazolium chloride as colorimetric indicator for drug susceptibility testing against nontuberculous mycobacteria.
作者信息
de Souza Mariana Quaresma, Bierhals Dienefer Venske, Reis Ana Julia, Chimara Erica, Vianna Júlia Silveira, von Groll Andrea, da Silva Pedro Almeida, Ramis Ivy Bastos
机构信息
Laboratório de Micobactérias, Núcleo de Pesquisa em Microbiologia Médica, Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil.
Núcleo de Tuberculose e Micobacterioses, Instituto Adolfo Lutz, São Paulo, Brazil.
出版信息
Diagn Microbiol Infect Dis. 2024 Jan;108(1):116096. doi: 10.1016/j.diagmicrobio.2023.116096. Epub 2023 Sep 24.
This study aimed to propose and evaluate a drug susceptibility testing (DST) using the 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator against Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii strains, main nontuberculous mycobacteria (NTM) of clinical relevance. Our results demonstrated that the assay using TTC and the broth microdilution method recommended by the Clinical and Laboratory Standards Institute had essential agreement above 91%, 92%, and 100%, for drugs tested against MABC, MAC, and M. kansasii strains, respectively. Categorical agreement above 91% was obtained for most drugs tested against MABC, except to cefoxitin (76.5%). For drugs tested against MAC and M. kansasii, categorical agreement above 92% and 100% was observed, respectively. TTC showed to be a promising colorimetric indicator of growth to be used in DST for NTM, allowing an easier reading of results.
本研究旨在提出并评估一种以2,3,5-三苯基氯化四氮唑（TTC）作为比色指示剂，针对脓肿分枝杆菌复合群（MABC）、鸟分枝杆菌复合群（MAC）和堪萨斯分枝杆菌菌株（具有临床相关性的主要非结核分枝杆菌（NTM））的药敏试验（DST）。我们的结果表明，使用TTC的试验与临床和实验室标准协会推荐的肉汤微量稀释法相比，针对MABC、MAC和堪萨斯分枝杆菌菌株测试的药物，其基本一致性分别高于91%、92%和100%。针对MABC测试的大多数药物，除头孢西丁（76.5%）外，分类一致性高于91%。针对MAC和堪萨斯分枝杆菌测试的药物，分类一致性分别观察到高于92%和100%。TTC显示出有望作为NTM药敏试验中生长的比色指示剂，使结果读取更容易。
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