Department of Neurology, The First Affiliated Hospital of Qiqihar Medical College, Qiqihar, Heilongjiang, China.
Growth Factors. 2024 Feb;42(1):13-23. doi: 10.1080/08977194.2023.2277755. Epub 2024 Jan 31.
Danggui blood-supplementing decoction (DBsD) is an herbal preparation treating several diseases including stroke. The present study sought to investigate the potential mechanism of DBsD in ischaemic stroke (IS) using network pharmacology, molecular docking, and cell experiment. Based on the protein-protein (PPI) network analysis, MAPK1 (0.51, 12), KNG1 (0.57, 28), and TNF (0.64, 39) were found with relatively good performance in degree and closeness centrality. The functional enrichment analysis revealed that DBsD contributed to IS-related biological processes, molecule function, and presynaptic/postsynaptic cellular components. Pathway enrichment indicated that DBsD might protect IS by modulating multi-signalling pathways including the sphingolipid signalling pathway. Molecular docking verified the stigmasterol-KNG1, bifendate-TNF, and formononetin-MAPK1 pairs. Cell experiments confirmed the involvement of KNG1 and sphingolipid signalling pathway in hippocampal neuronal cell apoptosis. This study showed that DBsD can protect neuronal cell injury after IS through multiple components, multiple targets, and multiple pathways.
当归补血汤（DBsD）是一种治疗多种疾病的草药制剂，包括中风。本研究旨在利用网络药理学、分子对接和细胞实验探讨 DBsD 治疗缺血性中风（IS）的潜在机制。基于蛋白质-蛋白质（PPI）网络分析，发现 MAPK1（0.51，12）、KNG1（0.57，28）和 TNF（0.64，39）在度和接近中心性方面表现相对较好。功能富集分析表明，DBsD 有助于与 IS 相关的生物学过程、分子功能和突触前/后细胞成分。通路富集表明，DBsD 可能通过调节包括鞘脂信号通路在内的多种信号通路来保护 IS。分子对接验证了豆甾醇-KNG1、双飞人-TNF 和芒柄花素-MAPK1 对。细胞实验证实了 KNG1 和鞘脂信号通路在海马神经元细胞凋亡中的作用。本研究表明，DBsD 可通过多种成分、多个靶点和多条途径保护 IS 后的神经元细胞损伤。
