[转录组测序用于鉴定兔静脉移植物再狭窄相关基因]

[Transcriptome sequencing for identification of the genes associated with restenosis of venous grafts in rabbits].

作者信息

Zhou H, Yang L, Xie S, Yang J, Zhang J

机构信息

Department of Thoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2023 Oct 20;43(10):1804-1809. doi: 10.12122/j.issn.1673-4254.2023.10.20.

DOI:10.12122/j.issn.1673-4254.2023.10.20

PMID:37933658

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10630209/

Abstract

OBJECTIVE

To identify the genes associated with venous graft restenosis in rabbits using transcriptome sequencing.

METHODS

Forty New Zealand rabbits were randomly divided into experimental group and control group, and in the experimental group, the left external jugular veins of the rabbits were engrafted to the left common carotid artery with continuous running suture; the rabbits in the control group received no operation. At 2 and 4 weeks after the operation, 10 rabbits from each group were euthanized and the venous grafts (in experimental group) or left external jugular vein (in control group) were harvested for measurement of the intima-media thickness using HE staining. RNA high-throughput sequencing (RNA-seq) was performed to identify the differentially expressed genes (DEGs) between the venous grafts and the control veins, and the biological functions of the DEGs were analyzed using GO and KEEG databases.

RESULTS

In the experimental group, intima-media thickening with increased extracellular matrix and vascular smooth muscle cell proliferation occurred in the venous grafts at 2 weeks and aggravated at 4 weeks after the operation. RNA high-throughput sequencing identified 1583 up-regulated genes and 608 down-regulated genes in the venous grafts in the experimental group, and GO and KEGG analysis of the DEGs pinpointed 10 hub genes, namely CD4, ZAP70, SYK, CD28, PIK3CD, CXCR4, CCR5, ITK, CCL5 and BTK.

CONCLUSION

CD4, ZAP70, SYK, CD28, PIK3CD, CXCR4, CCR5, ITK, CCL5 and BTK are probably the key genes associated with vein graft restenosis in rabbits.

摘要

目的

通过转录组测序鉴定与兔静脉移植物再狭窄相关的基因。

方法

40只新西兰兔随机分为实验组和对照组，实验组采用连续缝合将兔左侧颈外静脉移植至左侧颈总动脉；对照组不进行手术。术后2周和4周，每组分别处死10只兔子，取实验组的静脉移植物或对照组的左侧颈外静脉，采用苏木精-伊红染色测量内膜中层厚度。进行RNA高通量测序（RNA-seq）以鉴定静脉移植物与对照静脉之间的差异表达基因（DEGs），并使用GO和KEGG数据库分析DEGs的生物学功能。

结果

实验组术后2周静脉移植物出现内膜中层增厚，细胞外基质增加，血管平滑肌细胞增殖，4周时加重。RNA高通量测序鉴定出实验组静脉移植物中有1583个上调基因和608个下调基因，对DEGs进行GO和KEGG分析确定了10个核心基因，即CD4、ZAP70、SYK、CD28、PIK3CD、CXCR4、CCR5、ITK、CCL5和BTK。