探索SWI/SNF复合物亚基BAF60c在鱼类脂质代谢和炎症中的作用。
Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish.
作者信息
Sun Jie, Yan Qiuxin, Zhang Zhihao, Xu Ting, Gong Ye, Li Weijia, Mai Kangsen, Ai Qinghui
机构信息
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, People's Republic of China.
出版信息
iScience. 2023 Oct 13;26(11):108207. doi: 10.1016/j.isci.2023.108207. eCollection 2023 Nov 17.
Chromatin remodeling plays an important role in regulating gene transcription, in which chromatin remodeling complex is a crucial aspect. Brg1/Brm-associated factor 60c (BAF60c) subunit forms a bridge between chromatin remodeling complexes and transcription factors in mammals; hence, it has received extensive attention. However, the roles of BAF60c in fish remain largely unexplored. In this study, we identified BAF60c-interacting proteins by using HIS-pull-down and LC-MS/MS analysis in fish. Subsequently, the RNA-seq analysis was performed to identify the overall effects of BAF60c. Then, the function of BAF60c was verified through BAF60c knockdown and overexpression experiments. We demonstrated for the first time that BAF60c interacts with glucose-regulated protein 78 (GRP78) and regulates lipid metabolism, endoplasmic reticulum (ER) stress, and inflammation. Knockdown of BAF60c reduces fatty acid biosynthesis, ER stress, and inflammation. In conclusion, the results enriched BAF60c-interacting protein network and explored the function of BAF60c in lipid metabolism and inflammation in fish.
染色质重塑在调节基因转录中发挥着重要作用，其中染色质重塑复合体是一个关键方面。在哺乳动物中，Brg1/Brm相关因子60c（BAF60c）亚基在染色质重塑复合体和转录因子之间形成了一座桥梁；因此，它受到了广泛关注。然而，BAF60c在鱼类中的作用在很大程度上仍未得到探索。在本研究中，我们通过在鱼类中使用HIS下拉和液相色谱-串联质谱分析来鉴定与BAF60c相互作用的蛋白质。随后，进行RNA测序分析以确定BAF60c的总体影响。然后，通过BAF60c敲低和过表达实验验证了BAF60c的功能。我们首次证明BAF60c与葡萄糖调节蛋白78（GRP78）相互作用，并调节脂质代谢、内质网（ER）应激和炎症。敲低BAF60c可减少脂肪酸生物合成、ER应激和炎症。总之，这些结果丰富了BAF60c相互作用蛋白网络，并探索了BAF60c在鱼类脂质代谢和炎症中的功能。
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