Laboratory of Molecular Nutrition, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory of Molecular Nutrition, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Gene. 2024 Feb 5;894:147972. doi: 10.1016/j.gene.2023.147972. Epub 2023 Nov 7.
SREBPs, such as SREBP1 and SREBP2, were the key transcriptional factors regulating lipid metabolism. The processing of SREBPs involved many genes, such as scap, s1p, s2p, cideb. Here, we deciphered the full-length cDNA sequences of scap, srebp1, srebp2, s1p, s2p, cideb and cidec from yellow catfish Pelteobagrus fulvidraco. Their full-length cDNA sequences ranged from 1587 to 3884 bp, and their ORF length from 1191 to 2979 bp, encoding 396-992 amino acids. Some conservative domains were predicted, including the multiple transmembrane domains in SCAP, the bHLH-ZIP domain in SREBP1 and SREBP2, the ApoB binding region, ER targeting region and LD targeting region in CIDEb, the LD targeting region in the CIDEc, the conserved catalytic site and processing site in S1P, and the transmembrane helix domain in S2P. Their mRNA expression could be observed in the heart, spleen, liver, kidney, brain, muscle, intestine and adipose, but varied with tissues. The changes of their mRNA expression in responses to high-fat (HFD) and bile acid (BA) diets were also investigated in the brain, heart, intestine, kidney and spleen tissues. In the brain, HFD significantly increased the mRNA expression of seven genes (scap, srebp1, srebp2, s1p, s2p, cideb and cidec), and the BA attenuated the increase of scap, srebp1, srebp2, s1p, s2p, cideb and cidec mRNA expression induced by HFD. In the heart, HFD significantly increased the mRNA abundances of six genes (srebp1, srebp2, scap, s2p, cideb and cidec), and BA attenuated the increase of their mRNA abundances induced by HFD. In the intestine, HFD increased the cideb, s1p and s2p mRNA abundances, and BA attenuated the HFD-induced increment of their mRNA abundances. In the kidney, HFD significantly increased the scap, cidec and s1p mRNA expression, and BA diet attenuated the increment of their mRNA expression. In the spleen, HFD treatment increased the scap, srebp2, s1p and s2p mRNA expression, and BA diet attenuated HFD-induced increment of their mRNA expression. Taken together, our study elucidated the characterization, expression profiles and transcriptional response of seven lipid metabolic genes, which would serve as the good basis for the further exploration into their function and regulatory mechanism in fish.
SREBPs，如 SREBP1 和 SREBP2，是调节脂质代谢的关键转录因子。SREBPs 的加工涉及许多基因，如 scap、s1p、s2p、cideb。在这里，我们从黄颡鱼（Pelteobagrus fulvidraco）中破译了 scap、srebp1、srebp2、s1p、s2p、cideb 和 cidec 的全长 cDNA 序列。它们的全长 cDNA 序列长度为 1587 至 3884 bp，ORF 长度为 1191 至 2979 bp，编码 396-992 个氨基酸。预测到一些保守结构域，包括 scap 中的多个跨膜结构域、SREBP1 和 SREBP2 中的 bHLH-ZIP 结构域、CIDEb 中的 ApoB 结合区、ER 靶向区和 LD 靶向区、CIDEc 中的 LD 靶向区、S1P 中的保守催化位点和加工位点以及 S2P 中的跨膜螺旋结构域。它们的 mRNA 表达可在心脏、脾脏、肝脏、肾脏、大脑、肌肉、肠道和脂肪组织中观察到，但因组织而异。还研究了高脂（HFD）和胆汁酸（BA）饮食对它们在大脑、心脏、肠道、肾脏和脾脏组织中的 mRNA 表达的影响。在大脑中，HFD 显著增加了七个基因（scap、srebp1、srebp2、s1p、s2p、cideb 和 cidec）的 mRNA 表达，BA 减弱了 HFD 对 scap、srebp1、srebp2、s1p、s2p、cideb 和 cidec mRNA 表达的增加。在心脏中，HFD 显著增加了六个基因（srebp1、srebp2、scap、s2p、cideb 和 cidec）的 mRNA 丰度，BA 减弱了 HFD 对它们的 mRNA 丰度的增加。在肠道中，HFD 增加了 cideb、s1p 和 s2p 的 mRNA 丰度，BA 减弱了 HFD 对其 mRNA 丰度的增加。在肾脏中，HFD 显著增加了 scap、cidec 和 s1p 的 mRNA 表达，BA 饮食减弱了其 mRNA 表达的增加。在脾脏中，HFD 处理增加了 scap、srebp2、s1p 和 s2p 的 mRNA 表达，BA 饮食减弱了 HFD 诱导的这些基因的 mRNA 表达增加。总的来说，我们的研究阐明了七个脂质代谢基因的特征、表达谱和转录反应，这将为进一步探索鱼类中这些基因的功能和调控机制提供良好的基础。
