隐球菌抗原胶体金渗滤法检测在 HIV 阴性患者支气管肺泡灌洗液中的应用。
Performance of Cryptococcal Antigen Lateral Flow Assay in Bronchoalveolar Lavage Fluid in HIV-Negative Patients.
机构信息
Department of Respiratory and Critical Care Medicine, Ningbo, Zhejiang, China
Department of Respiratory and Critical Care Medicine, Ningbo, Zhejiang, China.
出版信息
Ann Clin Lab Sci. 2023 Sep;53(5):765-770. Epub 2023 Nov 9.
OBJECTIVE
We presented the performance of cryptococcal antigen lateral flow assay test using bronchoalveolar lavage fluid (BALF) samples in the HIV-negative Chinese population.
METHODS
From January 2019 to June 2022, cryptococcal antigen was detected in both serum and BALF samples from 113 patients with suspected pulmonary cryptococcosis.
RESULTS
49 patients were finally diagnosed with pulmonary cryptococcosis. The sensitivity of cryptococcal antigen lateral flow assay test in serum and BALF specimens from confirmed cases was 90.0% and 96.0%, respectively, and the specificity was 87.3% and 95.5%, respectively. When the diameter of the lung lesion was less than 15 mm, the antigen positivity rate of BALF was higher than that of serum. Moreover, the result of the cryptococcal antigen test was associated with the lymphocytes count of BALF.
CONCLUSION
Our data demonstrate that cryptococcal antigen Lateral Flow Assay for BALF specimens might contribute to the early diagnosis of pulmonary cryptococcosis.
目的
我们介绍了利用支气管肺泡灌洗液（BALF）样本检测隐球菌抗原的侧向流动检测试验在 HIV 阴性的中国人群中的表现。
方法
从 2019 年 1 月至 2022 年 6 月，对 113 例疑似肺隐球菌病的患者的血清和 BALF 样本进行了隐球菌抗原检测。
结果
最终有 49 例患者被确诊为肺隐球菌病。确诊病例中血清和 BALF 标本中隐球菌抗原侧向流动检测试验的敏感性分别为 90.0%和 96.0%，特异性分别为 87.3%和 95.5%。当肺部病变直径小于 15mm 时，BALF 中的抗原阳性率高于血清。此外，隐球菌抗原检测结果与 BALF 中的淋巴细胞计数有关。
结论
我们的数据表明，BALF 标本中的隐球菌抗原侧向流动检测试验可能有助于肺隐球菌病的早期诊断。
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