Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, China.
Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, China; Henan International Joint Laboratory of Children's Infectious Disease, China.
Indian J Med Microbiol. 2023 Nov-Dec;46:100435. doi: 10.1016/j.ijmmb.2023.100435. Epub 2023 Aug 1.
BACKGROUND/PURPOSE: As a major causative pathogen of community-acquired pneumonia, Mycoplasma pneumoniae (M. pneumoniae) can cause both upper and lower respiratory tract inflammation as well as extrapulmonary syndromes, especially in infants and the elderly. The emergence of macrolide-resistance has significant effects on the treatment of relevant diseases in children. This study aimed to analyze the genotypes and the macrolide resistance-associated mutations in M. pneumoniae sampled from the pediatric patients in Henan, China.
A segment of gene on the 23S rRNA was amplified and sequenced to detect the mutations related to macrolide resistance. Molecular typing was performed by the method named multiple locus variable-number tandem repeat analysis (MLVA) for macrolide-susceptible and macrolide-resistant specimens.
Among the M. pneumoniae-positive samples, 95.7% (111/116) had macrolide-resistant mutation, and all of them consisted of the A2063G mutation. There were only two MLVA types identified in this study, type 4-5-7-2 (51/92, 55.4%) and type 3-5-6-2 (41/92, 44.6%).
There was no correlation between MLVA types and macrolide resistance (P > 0.05).
背景/目的：肺炎支原体（M. pneumoniae）是社区获得性肺炎的主要病原体之一，可引起上、下呼吸道炎症及肺外综合征，尤其在婴幼儿和老年人中更为常见。大环内酯类耐药的出现对儿童相关疾病的治疗有重要影响。本研究旨在分析来自中国河南儿科患者的肺炎支原体样本的基因型和大环内酯类耐药相关突变。
扩增和测序 23S rRNA 基因的一段序列，以检测与大环内酯类耐药相关的突变。对大环内酯类敏感和耐药的标本采用多位点可变数目串联重复分析（MLVA）方法进行分子分型。
在肺炎支原体阳性样本中，95.7%（111/116）存在大环内酯类耐药突变，均为 A2063G 突变。本研究仅鉴定出两种 MLVA 类型，分别为 4-5-7-2 型（51/92，55.4%）和 3-5-6-2 型（41/92，44.6%）。
MLVA 类型与大环内酯类耐药之间无相关性（P>0.05）。
