Scott N W, Dow C S
J Gen Microbiol. 1986 Jul;132(7):1939-49. doi: 10.1099/00221287-132-7-1939.
DNA-dependent RNA polymerase has been isolated from Rhodomicrobium vannielii. Like those from other eubacteria, the enzyme contained four subunits: beta and beta prime (Mr 155,000), alpha (Mr 38,000), and sigma (Mr 98,000). Analysis by isoelectric focussing showed that both alpha and sigma had several forms with different isoelectric pH values. The enzyme was sensitive to rifampicin (5 ng rifampicin ml-1 gave 50% inhibition) and capable of specific promoter selection with DNA from R. vannielii, calf thymus and phage T7D111.
已从万氏红微菌中分离出依赖DNA的RNA聚合酶。与其他真细菌中的酶一样，该酶含有四个亚基：β和β′（分子量155,000）、α（分子量38,000）和σ（分子量98,000）。等电聚焦分析表明，α和σ均有几种具有不同等电pH值的形式。该酶对利福平敏感（5纳克/毫升利福平可产生50%的抑制作用），并且能够用来自万氏红微菌、小牛胸腺和噬菌体T7D111的DNA进行特异性启动子选择。
